Literature DB >> 10907080

Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR green II.

C Battaglia1, G Salani, C Consolandi, L R Bernardi, G De Bellis.   

Abstract

A simple, non-destructive procedure is described to determine the quality of DNA arrays before they are used. It consists of a preliminary staining step of the DNA microarray by using SYBR green II, a fluorophore with specific affinity for ssDNA, followed by a laser scan analysis. The surface quality, integrity and homogeneity of each DNA spot of the array can thus be assessed. After this preliminary control, which may avoid further analytical steps that lead to the waste of precious biological samples, a fully reversible staining procedure is performed that produces an array ready for subsequent use.

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Year:  2000        PMID： 10907080     DOI： 10.2144/00291st01

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  16 in total

1.  Three color cDNA microarrays: quantitative assessment through the use of fluorescein-labeled probes.

Authors:  Martin J Hessner; Xujing Wang; Katie Hulse; Lisa Meyer; Yan Wu; Steven Nye; Sun-Wei Guo; Soumitra Ghosh
Journal:  Nucleic Acids Res       Date:  2003-02-15       Impact factor: 16.971

2.  Microarray optimizations: increasing spot accuracy and automated identification of true microarray signals.

Authors:  Peter H Tran; Daniel A Peiffer; Yongchol Shin; Lauren M Meek; James P Brody; Ken W Y Cho
Journal:  Nucleic Acids Res       Date:  2002-06-15       Impact factor: 16.971

3.  Decoding randomly ordered DNA arrays.

Authors:  Kevin L Gunderson; Semyon Kruglyak; Michael S Graige; Francisco Garcia; Bahram G Kermani; Chanfeng Zhao; Diping Che; Todd Dickinson; Eliza Wickham; Jim Bierle; Dennis Doucet; Monika Milewski; Robert Yang; Chris Siegmund; Juergen Haas; Lixin Zhou; Arnold Oliphant; Jian-Bing Fan; Steven Barnard; Mark S Chee
Journal:  Genome Res       Date:  2004-04-12       Impact factor: 9.043

Review 4.  DNA microarrays--techniques and applications in microbial systems.

Authors:  T Majtán; G Bukovská; J Timko
Journal:  Folia Microbiol (Praha)       Date:  2004       Impact factor: 2.099

5.  Diagnostic oligonucleotide microarray fingerprinting of Bacillus isolates.

Authors:  Darrell P Chandler; Oleg Alferov; Boris Chernov; Don S Daly; Julia Golova; Alexander Perov; Miroslava Protic; Richard Robison; Matthew Schipma; Amanda White; Alan Willse
Journal:  J Clin Microbiol       Date:  2006-01       Impact factor: 5.948

6.  Simultaneous detection of precore/basal core promoter mutations in hepatitis B virus using arrayed primer extension.

Authors:  Wai-Yan Ha; Chi-Chiu Lau; Patrick Y K Yue; Kaman K M Hung; Kelvin Chan; Siu-Hon Chui; Albert K K Chui; Wing-Cheong Yam; Ricky N S Wong
Journal:  Mol Diagn Ther       Date:  2006       Impact factor: 4.074

7.  Autoinducer-2-regulated genes in Streptococcus mutans UA159 and global metabolic effect of the luxS mutation.

Authors:  Helena Sztajer; André Lemme; Ramiro Vilchez; Stefan Schulz; Robert Geffers; Cindy Ying Yin Yip; Celine M Levesque; Dennis G Cvitkovitch; Irene Wagner-Döbler
Journal:  J Bacteriol       Date:  2007-11-02       Impact factor: 3.490

8.  Analysis of the quality of contact-pin fabricated oligonucleotide microarrays.

Authors:  Amy X Yang; Josef Mejido; Bhaskar Bhattacharya; David Petersen; Jing Han; Ernest S Kawasaki; Raj K Puri
Journal:  Mol Biotechnol       Date:  2006-11       Impact factor: 2.695

9.  Development of a DNA microarray to detect antimicrobial resistance genes identified in the National Center for Biotechnology Information database.

Authors:  Jonathan G Frye; Rebecca L Lindsey; Gaelle Rondeau; Steffen Porwollik; Fred Long; Michael McClelland; Charlene R Jackson; Mark D Englen; Richard J Meinersmann; Mark E Berrang; Johnnie A Davis; John B Barrett; Jennifer B Turpin; Sutawee N Thitaram; Paula J Fedorka-Cray
Journal:  Microb Drug Resist       Date:  2010-03       Impact factor: 3.431

10.  Inhibition of CCN6 (Wnt-1-induced signaling protein 3) down-regulates E-cadherin in the breast epithelium through induction of snail and ZEB1.

Authors:  Wei Huang; Yanhong Zhang; Sooryanarayana Varambally; Arul M Chinnaiyan; Mousumi Banerjee; Sofia D Merajver; Celina G Kleer
Journal:  Am J Pathol       Date:  2008-03-05       Impact factor: 4.307
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