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Literature DB >> 10869088

A PhoP-regulated outer membrane protease of Salmonella enterica serovar typhimurium promotes resistance to alpha-helical antimicrobial peptides.

T Guina¹, E C Yi, H Wang, M Hackett, S I Miller.

Abstract

The outer membrane protein contents of Salmonella enterica serovar Typhimurium strains with PhoP/PhoQ regulon mutations were compared by two-dimensional gel electrophoresis. At least 26 species of outer membrane proteins (OMPs) were identified as being regulated by PhoP/PhoQ activation. One PhoP/PhoQ-activated OMP was identified by semiautomated tandem mass spectrometry coupled with electronic database searching as PgtE, a member of the Escherichia coli OmpT and Yersinia pestis Pla family of outer membrane proteases. Salmonella PgtE expression promoted resistance to alpha-helical cationic antimicrobial peptides (alpha-CAMPs). Strains expressing PgtE cleaved C18G, an 18-residue alpha-CAMP present in culture medium, indicating that protease activity is likely to be the mechanism of OmpT-mediated resistance to alpha-CAMPs. PhoP/PhoQ did not regulate the transcription or export of PgtE, indicating that another PhoP/PhoQ-dependent mechanism is required for PgtE outer membrane localization. PgtE is a posttranscriptionally regulated component of the PhoP/PhoQ regulon that contributes to Salmonella resistance to innate immunity.

Entities: Chemical Gene Species

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Year: 2000 PMID： 10869088 PMCID： PMC94595 DOI： 10.1128/JB.182.14.4077-4086.2000

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

54 in total

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116 in total

Review 1. The pleiotropic two-component regulatory system PhoP-PhoQ.

Authors: E A Groisman
Journal: J Bacteriol Date: 2001-03 Impact factor: 3.490

2. OmpT outer membrane proteases of enterohemorrhagic and enteropathogenic Escherichia coli contribute differently to the degradation of human LL-37.

Authors: Jenny-Lee Thomassin; John R Brannon; Bernard F Gibbs; Samantha Gruenheid; Hervé Le Moual
Journal: Infect Immun Date: 2011-12-05 Impact factor: 3.441

A PhoP-regulated outer membrane protease of Salmonella enterica serovar typhimurium promotes resistance to alpha-helical antimicrobial peptides.

1. Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and phi80 transducing phages.

2. A Salmonella typhimurium virulence protein is similar to a Yersinia enterocolitica invasion protein and a bacteriophage lambda outer membrane protein.

3. Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues: identity of protease VII and OmpT.

4. Identification and nucleotide sequence of the activator gene of the externally induced phosphoglycerate transport system of Salmonella typhimurium.

5. Protein sequencing by tandem mass spectrometry.

6. Complete nucleotide sequence and deduced amino acid sequence of the ompT gene of Escherichia coli K-12.

7. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.

8. Construction and properties of a new cloning vehicle, allowing direct screening for recombinant plasmids.

9. The plasmid F OmpP protease, a homologue of OmpT, as a potential obstacle to E. coli-based protein production.

10. TnphoA: a transposon probe for protein export signals.

Review 1. The pleiotropic two-component regulatory system PhoP-PhoQ.

2. OmpT outer membrane proteases of enterohemorrhagic and enteropathogenic Escherichia coli contribute differently to the degradation of human LL-37.

3. Prevalence, development, and molecular mechanisms of bacteriocin resistance in Campylobacter.

Review 4. Molecular basis of bacterial outer membrane permeability revisited.

Review 5. Endogenous production of antimicrobial peptides in innate immunity and human disease.

6. Phenotype microarray analysis of Escherichia coli K-12 mutants with deletions of all two-component systems.

7. The PhoP-PhoQ two-component regulatory system of Photorhabdus luminescens is essential for virulence in insects.

8. Antimicrobial peptides activate the Rcs regulon through the outer membrane lipoprotein RcsF.

9. Infection with an avirulent phoP mutant of Neisseria meningitidis confers broad cross-reactive immunity.

10. The Erwinia chrysanthemi 3937 PhoQ sensor kinase regulates several virulence determinants.