Literature DB >> 10830134

Expression and purification of rhodopsin and its mutants from stable mammalian cell lines: application to NMR studies.

P J Reeves1, J Klein-Seetharaman, E V Getmanova, M Eilers, M C Loewen, S O Smith, H G Khorana.   

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Year:  1999        PMID： 10830134     DOI： 10.1042/bst0270950

Source DB:  PubMed          Journal:  Biochem Soc Trans        ISSN： 0300-5127            Impact factor:   5.407


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  11 in total

1.  Solution 19F nuclear Overhauser effects in structural studies of the cytoplasmic domain of mammalian rhodopsin.

Authors:  M C Loewen; J Klein-Seetharaman; E V Getmanova; P J Reeves; H Schwalbe; H G Khorana
Journal:  Proc Natl Acad Sci U S A       Date:  2001-04-24       Impact factor: 11.205

2.  Differential dynamics in the G protein-coupled receptor rhodopsin revealed by solution NMR.

Authors:  Judith Klein-Seetharaman; Naveena V K Yanamala; Fathima Javeed; Philip J Reeves; Elena V Getmanova; Michele C Loewen; Harald Schwalbe; H Gobind Khorana
Journal:  Proc Natl Acad Sci U S A       Date:  2004-02-27       Impact factor: 11.205

3.  Large-scale expression and purification of a G-protein-coupled receptor for structure determination -- an overview.

Authors:  Reinhard Grisshammer; Jim F White; Loc B Trinh; Joseph Shiloach
Journal:  J Struct Funct Genomics       Date:  2005

4.  Structure and function in rhodopsin: a tetracycline-inducible system in stable mammalian cell lines for high-level expression of opsin mutants.

Authors:  Philip J Reeves; Jong-Myoung Kim; H Gobind Khorana
Journal:  Proc Natl Acad Sci U S A       Date:  2002-10-07       Impact factor: 11.205

5.  Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line.

Authors:  Philip J Reeves; Nico Callewaert; Roland Contreras; H Gobind Khorana
Journal:  Proc Natl Acad Sci U S A       Date:  2002-10-07       Impact factor: 11.205

6.  Solution NMR spectroscopy of [alpha -15N]lysine-labeled rhodopsin: The single peak observed in both conventional and TROSY-type HSQC spectra is ascribed to Lys-339 in the carboxyl-terminal peptide sequence.

Authors:  J Klein-Seetharaman; P J Reeves; M C Loewen; E V Getmanova; J Chung; H Schwalbe; P E Wright; H G Khorana
Journal:  Proc Natl Acad Sci U S A       Date:  2002-03-19       Impact factor: 11.205

Review 7.  Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.

Authors:  Willem J de Grip; Srividya Ganapathy
Journal:  Front Chem       Date:  2022-06-22       Impact factor: 5.545

Review 8.  Purification of recombinant G-protein-coupled receptors.

Authors:  Reinhard Grisshammer
Journal:  Methods Enzymol       Date:  2009       Impact factor: 1.600

9.  Dipolar assisted rotational resonance NMR of tryptophan and tyrosine in rhodopsin.

Authors:  Evan Crocker; Ashish B Patel; Markus Eilers; Shobini Jayaraman; Elena Getmanova; Philip J Reeves; Martine Ziliox; H Gobind Khorana; Mordechai Sheves; Steven O Smith
Journal:  J Biomol NMR       Date:  2004-05       Impact factor: 2.835

10.  G Protein-Coupled Receptors Contain Two Conserved Packing Clusters.

Authors:  Omar B Sanchez-Reyes; Aidan L G Cooke; Dale B Tranter; Dawood Rashid; Markus Eilers; Philip J Reeves; Steven O Smith
Journal:  Biophys J       Date:  2017-06-06       Impact factor: 3.699
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