Literature DB >> 10793714

Some general methods of preparing affinity columns.

P V Sundaram.   

Abstract

Some general methods of covalent coupling of nucleotides, especially derivatized nucleotides, polynucleotides and cofactors to insoluble polymers are described in this paper. Wherever necessary individual methods also carry some information on the binding of enzymes to the same polymers to serve as a guide to the efficiency of the coupling methods.

Entities:  

Mesh：

Substances：

Year:  1974        PMID： 10793714      PMCID： PMC343438          DOI： 10.1093/nar/1.11.1587

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Preparation and properties of urease chemically attached to nylon tube.

Authors:  P V. Sundaram; W E. Hornby
Journal:  FEBS Lett       Date:  1970-10       Impact factor: 4.124

2.  SYNTHESIS OF NUCLEOTIDE ANHYDRIDES BY ANION EXCHANGE.

Authors:  A M MICHELSON
Journal:  Biochim Biophys Acta       Date:  1964-09-11

3.  Some parameters relevant to affinity chromatography on immobilized nucleotides.

Authors:  C R Lowe; M J Harvey; D B Craven; P D Dean
Journal:  Biochem J       Date:  1973-07       Impact factor: 3.857

4.  Some studies on insolubilized nicotinamide nucleotides.

Authors:  P D Dean; C R Lowe
Journal:  Biochem J       Date:  1972-04       Impact factor: 3.857

5.  Studies on the reaction mechanism of soybean callus succinyl CoA synthetase. Phosphorylation of the enzyme on immobilized adenosine-triphosphate and enzyme attached to a solid support.

Authors:  E A Wider de Xifra; S Mendiara; A M Batlle
Journal:  FEBS Lett       Date:  1972-11-01       Impact factor: 4.124

6.  Synthesis of ATP- and dATP-substituted sepharoses and their application in the purification of phage-T4-induced ribonucleotide reductase.

Authors:  O Berglund; F Eckstein
Journal:  Eur J Biochem       Date:  1972-08-04

7.  Preparation of sepharose-bound poly (rI:rC).

Authors:  A F Wagner; R L Bugianesi; T Y Shen
Journal:  Biochem Biophys Res Commun       Date:  1971-10-01       Impact factor: 3.575

8.  Covalent attachment of nucleic acids to agarose for affinity chromatography.

Authors:  M S Poonian; A J Schlabach; A Weissbach
Journal:  Biochemistry       Date:  1971-02-02       Impact factor: 3.162

9.  A reinvestigation of the mixed carbonic anhydride method of peptide synthesis.

Authors:  G W Anderson; J E Zimmerman; F M Callahan
Journal:  J Am Chem Soc       Date:  1967-09-13       Impact factor: 15.419

10.  General ligands in affinity chromatography. Cofactor-substrate elution of enzymes bound to the immobilized nucleotides adenosine 5'-monophosphate and nicotinamide-adenine dinucleotide.

Authors:  K Mosbach; H Guilford; R Ohlsson; M Scott
Journal:  Biochem J       Date:  1972-05       Impact factor: 3.857
View more
  6 in total

1.  Preparation and properties of nylon-tube-supported nicotinamide--adenine dinucleotide kinase.

Authors:  P V Sundaram; D K Apps
Journal:  Biochem J       Date:  1977-02-01       Impact factor: 3.857

2.  Preparation and properties of a poly A resin and its use in the isolation of naturally occurring poly U sequences.

Authors:  S Venkatesan; H Nakazato; M Edmonds
Journal:  Nucleic Acids Res       Date:  1976-08       Impact factor: 16.971

3.  Agarose linked adenosine and guanosine-5'-monophosphate; a new general method for the coupling of ribonucleotides to polymers through their cis-diols.

Authors:  F Seela; S Waldek
Journal:  Nucleic Acids Res       Date:  1975-12       Impact factor: 16.971

4.  The reversible immobilization of proteins on nylon activated through the formation of a substituted imidoester, and its unusual properties.

Authors:  P V Sundaram
Journal:  Biochem J       Date:  1979-11-01       Impact factor: 3.857

5.  Preparation of polydeoxynucleotides linked to a solid support by coupling CNBr-activated cellulose with 5'-NH2-terminated oligo and poly(pdT)'s.

Authors:  L Clerici; F Campagnari; J F de Rooij; J H van Boom
Journal:  Nucleic Acids Res       Date:  1979-01       Impact factor: 16.971

6.  A facile method for the preparation of N6-substituted ATP-Sepharose.

Authors:  F Eckstein; M Goumet; R Wetzel
Journal:  Nucleic Acids Res       Date:  1975-10       Impact factor: 16.971
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.