Literature DB >> 1187346

A facile method for the preparation of N6-substituted ATP-Sepharose.

F Eckstein, M Goumet, R Wetzel.   

Abstract

The attachment of 6-methylsulfonylpurineribose 5'-triphosphate to AH-Sepharose 4B as a facile method for the preparation of ATP-Sepharose is described. This reaction is compared to the coupling of 6-chloropurineriboside 5'-triphosphate as well as N(6)-aminocaproyl-ATP. An improved synthesis of purineriboside 5'-phosphates which does not require purification by column chromatography is also described.

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Year:  1975        PMID： 1187346      PMCID： PMC343544          DOI： 10.1093/nar/2.10.1771

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Synthesis and reactions of 6-methylsulfonyl-9-beta-D-ribofuranosylpurine.

Authors:  R Wetzel; F Eckstein
Journal:  J Org Chem       Date:  1975-03-07       Impact factor: 4.354

2.  SYNTHESIS OF NUCLEOTIDE ANHYDRIDES BY ANION EXCHANGE.

Authors:  A M MICHELSON
Journal:  Biochim Biophys Acta       Date:  1964-09-11

3.  ATP-and dATP-substituted agaroses and the purification of ribonucleotide reductases.

Authors:  O Berglund; F Eckstein
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

4.  Conversion of protein kinase to a cyclic AMP independent form by affinity chromatography on N 6 -caproyl 3',5'-cyclic adenosine monophosphate-sepharose.

Authors:  M Wilchek; Y Salomon; M Lowe; Z Selinger
Journal:  Biochem Biophys Res Commun       Date:  1971-12-03       Impact factor: 3.575

5.  N6-immobilized 5 -AMP and NAD+: preparations and applications.

Authors:  M J Harvey; D B Craven; C R Lowe; P D Dean
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

6.  AMP and NAD as "General Ligands".

Authors:  K Mosbach
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

7.  ATP-analogue-bound gel matrix and its use as an affinity adsorbent for Na+, K+-ATPase.

Authors:  B H Anderton; F W Hulla; H Fasold; H A White
Journal:  FEBS Lett       Date:  1973-12-01       Impact factor: 4.124

8.  A simple general method for the preparation of "6-immobilized" analogues of AMP, ATP, NAD and of other adenine-containing compounds for affinity chromatography.

Authors:  S Barry; P O'Carra
Journal:  FEBS Lett       Date:  1973-12-01       Impact factor: 4.124

9.  Immobilized nucleotides for affinity chromatography.

Authors:  M Wilchek; R Lamed
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

10.  Preparation of adenosine nucleotide derivatives suitable for affinity chromatography.

Authors:  I P Trayer; H R Trayer; D P Small; R C Bottomley
Journal:  Biochem J       Date:  1974-06       Impact factor: 3.857
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  2 in total

1.  Agarose linked adenosine and guanosine-5'-monophosphate; a new general method for the coupling of ribonucleotides to polymers through their cis-diols.

Authors:  F Seela; S Waldek
Journal:  Nucleic Acids Res       Date:  1975-12       Impact factor: 16.971

2.  The immobilization of adenine nucleotides on polysaccharides by using glutaraldehyde coupling and borohydride reduction.

Authors:  P Gacesa; W J Whish
Journal:  Biochem J       Date:  1978-10-01       Impact factor: 3.857
  2 in total

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