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Literature DB >> 10718611

Determination of h2J(NN) and h1J(HN) coupling constants across Watson-Crick base pairs in the Antennapedia homeodomain-DNA complex using TROSY.

K Pervushin¹, C Fernández, R Riek, A Ono, M Kainosho, K Wüthrich.

Abstract

This paper describes NMR measurements of 15N-15N and 1H-15N scalar couplings across hydrogen bonds in Watson-Crick base pairs, h2J(NN) and h1J(HN), in a 17 kDa Antennapedia homeodomain-DNA complex. A new NMR experiment is introduced which relies on zero-quantum coherence-based transverse relaxation-optimized spectroscopy (ZQ-TROSY) and enables measurements of h1J(HN) couplings in larger molecules. The h2JNN and h1J(HN) couplings open a new avenue for comparative studies of DNA duplexes and other forms of nucleic acids free in solution and in complexes with proteins, drugs or possibly other classes of compounds.

Entities: Chemical

Mesh：

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Year: 2000 PMID： 10718611 DOI： 10.1023/a:1008367405025

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

12 in total

1. The 3D NOESY-[(1)H,(15)N,(1)H]-ZQ-TROSY NMR experiment with diagonal peak suppression.

Authors: K V Pervushin; G Wider; R Riek; K Wüthrich
Journal: Proc Natl Acad Sci U S A Date: 1999-08-17 Impact factor: 11.205

2. Comparison of X-ray and NMR structures for the Antennapedia homeodomain-DNA complex.

Authors: E Fraenkel; C O Pabo
Journal: Nat Struct Biol Date: 1998-08

3. Adiabatic mixing in the liquid state.

Authors: E Kupce; P Schmidt; M Rance; G Wagner
Journal: J Magn Reson Date: 1998-12 Impact factor: 2.229

4. Conformational changes of the BS2 operator DNA upon complex formation with the Antennapedia homeodomain studied by NMR with 13C/15N-labeled DNA.

Authors: C Fernández; T Szyperski; M Billeter; A Ono; H Iwai; M Kainosho; K Wüthrich
Journal: J Mol Biol Date: 1999-09-24 Impact factor: 5.469

5. Single Transition-to-single Transition Polarization Transfer (ST2-PT) in [15N,1H]-TROSY.

Authors: K V Pervushin; G Wider; K Wüthrich
Journal: J Biomol NMR Date: 1998-08 Impact factor: 2.835

6. NMR with (13)C, (15)N-doubly-labeled DNA: The shape Antennapedia homeodomain complex with a 14-mer DNA duplex.

Authors: C Fernández; T Szyperski; A Ono; H Iwai; S Tate; M Kainosho; K Wüthrich
Journal: J Biomol NMR Date: 1998-07 Impact factor: 2.835

7. Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution.

Authors: K Pervushin; R Riek; G Wider; K Wüthrich
Journal: Proc Natl Acad Sci U S A Date: 1997-11-11 Impact factor: 11.205

8. Quantitative measurement of small through-hydrogen-bond and 'through-space' 1H-113Cd and 1H-199Hg J couplings in metal-substituted rubredoxin from Pyrococcus furiosus.

Authors: P R Blake; B Lee; M F Summers; M W Adams; J B Park; Z H Zhou; A Bax
Journal: J Biomol NMR Date: 1992-09 Impact factor: 2.835

9. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions.

Authors: M Piotto; V Saudek; V Sklenár
Journal: J Biomol NMR Date: 1992-11 Impact factor: 2.835

10. Isolation and sequence-specific DNA binding of the Antennapedia homeodomain.

Authors: M Müller; M Affolter; W Leupin; G Otting; K Wüthrich; W J Gehring
Journal: EMBO J Date: 1988-12-20 Impact factor: 11.598

7 in total

1. Modeling 2hJiso(N, N) in nucleic acid base pairs: ab initio characterization of the 2hJ(N, N) tensor in the methyleneimine dimer as a function of hydrogen bond geometry.

Authors: D L Bryce; R E Wasylishen
Journal: J Biomol NMR Date: 2001-04 Impact factor: 2.835

2. Improved TROSY-HNCA experiment with suppression of conformational exchange induced relaxation.

Authors: K Pervushin; V Gallius; C Ritter
Journal: J Biomol NMR Date: 2001-10 Impact factor: 2.835

3. Studies of physicochemical properties of N-H...N hydrogen bonds in DNA, using selective 15N-labeling and direct 15N 1D NMR.

Authors: C Kojima; A Ono; M Kainosho
Journal: J Biomol NMR Date: 2000-11 Impact factor: 2.835

4. The use of TROSY for detection and suppression of conformational exchange NMR line broadening in biological macromolecules.

Authors: K Pervushin
Journal: J Biomol NMR Date: 2001-07 Impact factor: 2.835