| Literature DB >> 10675609 |
C J Pachuk1, M Samuel, J A Zurawski, L Snyder, P Phillips, C Satishchandran.
Abstract
A novel DNA assembly method, chain reaction cloning (CRC), is described. CRC enables the ordered assembly of multiple DNA fragments in a single step. The power of the technique was demonstrated by the directed in vitro assembly of a plasmid comprised of six DNA fragments from a pool of 12 available fragments. The odds of obtaining the correct plasmid clone in a single step, using conventional techniques, is less than 1 in 191000000. Using CRC, the desired plasmid was recovered at a frequency of one in two. Ligation is no longer the rate limiting step in cloning, and limitless possibilities exist for the reconstruction of complex genomes.Mesh：
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Year: 2000 PMID： 10675609 DOI： 10.1016/s0378-1119(99)00508-9
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688