Literature DB >> 10664971

Efficient subtyping of Yersinia enterocolitica bioserotype 4/O:3 with pulsed-field gel electrophoresis.

M Fredriksson-Ahomaa1, T Autio, H Korkeala.   

Abstract

The purpose of this study was to evaluate the efficiency of pulsed-field gel electrophoresis (PFGE) for differentiation of isolates of Yersinia enterocolitica bioserotype 4/O:3 recovered from pig tongues at retail level in the Helsinki area during October and November of 1996. NotI generated 15 different PFGE patterns for 128 isolates. The discriminatory index did not exceed 74% due to the presence of two predominant PFGE-types, NA1 (58/128) and NB1 (25/128). After preliminary investigations with 35 enzymes, ApaI, XbaI, XhoI and SpeI were chosen for further characterization of the isolates. The discriminatory index increased from 74% to 93% and the number of different genotypes from 15 to 30 when isolates with the same PFGE pattern with NotI were further characterized with ApaI and XhoI, indicating that PFGE can be an efficient technique for characterization of bioserotype 4/O:3.

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Year:  1999        PMID： 10664971     DOI： 10.1046/j.1365-2672.1999.00625.x

Source DB:  PubMed          Journal:  Lett Appl Microbiol        ISSN： 0266-8254            Impact factor:   2.858


  12 in total

1.  Clonal dissemination of Yersinia enterocolitica strains with various susceptibilities to nalidixic acid.

Authors:  Javier Sánchez-Céspedes; Margarita M Navia; Rocío Martínez; Beatriz Orden; Rosario Millán; Joaquín Ruiz; Jordi Vila
Journal:  J Clin Microbiol       Date:  2003-04       Impact factor: 5.948

2.  Epidemiological study of resistance to nalidixic acid and other antibiotics in clinical Yersinia enterocolitica O:3 isolates.

Authors:  S Capilla; P Goñi; M C Rubio; J Castillo; L Millán; P Cerdá; J Sahagún; C Pitart; A Beltrán; R Gómez-Lus
Journal:  J Clin Microbiol       Date:  2003-10       Impact factor: 5.948

3.  Application of fluorescent amplified fragment length polymorphism for comparison of human and animal isolates of Yersinia enterocolitica.

Authors:  Catherine Fearnley; Stephen L W On; Branko Kokotovic; Georgina Manning; Tom Cheasty; Diane G Newell
Journal:  Appl Environ Microbiol       Date:  2005-09       Impact factor: 4.792

4.  Identification and characterization of pathogenic Yersinia enterocolitica isolates by PCR and pulsed-field gel electrophoresis.

Authors:  S Thisted Lambertz; M-L Danielsson-Tham
Journal:  Appl Environ Microbiol       Date:  2005-07       Impact factor: 4.792

5.  Development of multiple-locus variable-number tandem-repeat analysis for Yersinia enterocolitica subsp. palearctica and its application to bioserogroup 4/O3 subtyping.

Authors:  Rafał Gierczyński; Andrey Golubov; Heinrich Neubauer; Jeannette N Pham; Alexander Rakin
Journal:  J Clin Microbiol       Date:  2007-06-06       Impact factor: 5.948

6.  Multiple-locus variable-number tandem-repeat analysis in genotyping Yersinia enterocolitica strains from human and porcine origins.

Authors:  S Virtanen; R Laukkanen-Ninios; P Ortiz Martínez; A Siitonen; M Fredriksson-Ahomaa; H Korkeala
Journal:  J Clin Microbiol       Date:  2013-05-01       Impact factor: 5.948

7.  virF-positive Yersinia pseudotuberculosis and Yersinia enterocolitica found in migratory birds in Sweden.

Authors:  Taina Niskanen; Jonas Waldenström; Maria Fredriksson-Ahomaa; Björn Olsen; Hannu Korkeala
Journal:  Appl Environ Microbiol       Date:  2003-08       Impact factor: 4.792

8.  Multilocus variable-number tandem-repeat analysis, pulsed-field gel electrophoresis, and antimicrobial susceptibility patterns in discrimination of sporadic and outbreak-related strains of Yersinia enterocolitica.

Authors:  Leila M Sihvonen; Susanna Toivonen; Kaisa Haukka; Markku Kuusi; Mikael Skurnik; Anja Siitonen
Journal:  BMC Microbiol       Date:  2011-02-25       Impact factor: 3.605

9.  Rodents on pig and chicken farms - a potential threat to human and animal health.

Authors:  Annette Backhans; Claes Fellström
Journal:  Infect Ecol Epidemiol       Date:  2012-02-17

10.  Genotypic Characterization of Yersinia enterocolitica Biotype 4/O:3 Isolates from Pigs and Slaughterhouses Using SE-AFLP, ERIC-PCR, and PFGE.

Authors:  Renata Paixão; Luisa Zanolli Moreno; Débora Dirani Sena de Gobbi; Daniele Cristine Raimundo; Thais Sebastiana Porfida Ferreira; Maria Garcia Spindola; Ernesto Hofer; Cristhiane Moura Falavina Dos Reis; Maria Helena Matté; Andrea Micke Moreno
Journal:  J Pathog       Date:  2013-05-30
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