Biological fixation of the graft within bone after anterior cruciate ligament reconstruction in rabbits: effects of the duration of postoperative immobilization.

To investigate the effects of the duration of postoperative immobilization on the biological fixation of the graft within bone after anterior cruciate ligament (ACL) reconstruction, we performed ACL reconstruction in 27 rabbits, which were divided into five groups - no immobilization and 1-, 2-, 4-, and 6-week immobilization. All animals were killed 6 weeks postoperatively, and the graft-bone tunnel interface was examined biomechanically and histologically. In the groups with immobilization, in tensile failure tests, most specimens failed through the intraarticular portion of the grafts. There was no significant difference in the maximum load between these groups. In the no-immobilization group, all specimens failed through pulling of the grafts out of the femoral tunnel, with a lower maximum load than in the other groups. Histologically, new bone formation and occasional collagen fiber continuity were observed at the interface; which consisted of a fibrous tissue. In the no-immobilization group, the graft was partly separated from the fibrous tissue covering the bony wall. It is concluded that no immobilization delays the biological fixation process in the bone tunnel after ACL reconstruction, and that a certain period of immobilization is necessary for the fixation to proceed smoothly.