M Blake1, J Garrisi, G Tomkin, J Cohen. 1. The Institute for Reproductive Medicine and Science of Saint Barnabas, West Orange, New Jersey, USA. MarlenaBlake@embryos.net
Abstract
OBJECTIVE: To evaluate the effects of sperm placement during ICSI relative to the M-II spindle location on fertilization and preimplantation development. DESIGN: Retrospective analysis of oocyte fertilization and embryo development with respect to sperm deposition site during ICSI. SETTING: A program of IVF-ET. PATIENT(S): Seven hundred seventy-six patients. INTERVENTION(S): Egg quality, sperm deposition site, and polar-body position were recorded during ICSI; fertilization was assessed on day 1; embryo development was evaluated on days 2 and 3. MAIN OUTCOME MEASURE(S): Fertilization, embryo development, and implantation rates. RESULT(S): Normal fertilization is not affected by polar-body orientation, with the exception of a significantly lower fertilization rate from a 9 o'clock polar-body orientation. Injections with the polar-body positioned at 7 or 11 o'clock result in the greatest number of high-quality embryos, significantly more than the adjacent 6 or 12 o'clock polar-body orientations and irrespective of oocyte anomaly frequency. Embryos originating from the 7 or 11 o'clock polar-body category implant at a higher rate, although the data are not significant. CONCLUSION(S): The placement of the sperm during ICSI relative to the presumed location of the meiotic spindle significantly impacts fertilization and high-quality embryo development. Sperm deposition in the M-II spindle area should be avoided. It appears that development, and not fertilization, is improved by decreasing the distance between the sperm cell and the spindle.
OBJECTIVE: To evaluate the effects of sperm placement during ICSI relative to the M-II spindle location on fertilization and preimplantation development. DESIGN: Retrospective analysis of oocyte fertilization and embryo development with respect to sperm deposition site during ICSI. SETTING: A program of IVF-ET. PATIENT(S): Seven hundred seventy-six patients. INTERVENTION(S): Egg quality, sperm deposition site, and polar-body position were recorded during ICSI; fertilization was assessed on day 1; embryo development was evaluated on days 2 and 3. MAIN OUTCOME MEASURE(S): Fertilization, embryo development, and implantation rates. RESULT(S): Normal fertilization is not affected by polar-body orientation, with the exception of a significantly lower fertilization rate from a 9 o'clock polar-body orientation. Injections with the polar-body positioned at 7 or 11 o'clock result in the greatest number of high-quality embryos, significantly more than the adjacent 6 or 12 o'clock polar-body orientations and irrespective of oocyte anomaly frequency. Embryos originating from the 7 or 11 o'clock polar-body category implant at a higher rate, although the data are not significant. CONCLUSION(S): The placement of the sperm during ICSI relative to the presumed location of the meiotic spindle significantly impacts fertilization and high-quality embryo development. Sperm deposition in the M-II spindle area should be avoided. It appears that development, and not fertilization, is improved by decreasing the distance between the sperm cell and the spindle.
Authors: Iman Halvaei; Mohammad Ali Khalili; Mohammad Hossein Razi; Stefania A Nottola Journal: J Assist Reprod Genet Date: 2012-05-30 Impact factor: 3.412
Authors: A De Vos; M Abraham; N Franceus; P Haentjens; H Tournaye; G Verheyen; H Van de Velde Journal: J Assist Reprod Genet Date: 2015-05-01 Impact factor: 3.412