| Literature DB >> 10622635 |
K Tanabe1, N Sakihama, O Kaneko, A Saito-Ito, M Kimura.
Abstract
Merozoite surface protein-1 (MSP-1) of Plasmodium falciparum is a strong malaria vaccine candidate. However, MSP-1 exhibits extensive antigenic polymorphism, an issue which may compromise the development of effective vaccine based on this molecule. Since polymorphic nature of MSP-1 has not been fully understood in endemic areas of malaria, variation of the MSP-1 gene (Msp-1) must be studied in detail in natural parasite populations. Here, a PCR-based method for determination of P. falciparum Msp-1 haplotype is described, which can detect up to 24 different haplotypes per infected person. The method can be applied to various purposes of molecular epidemiology of not only Msp-1 haplotype but the genetic structure of P. falciparum populations.Entities:
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Year: 1998 PMID： 10622635
Source DB: PubMed Journal: Tokai J Exp Clin Med ISSN： 0385-0005