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Literature DB >> 1059083

Synthesis of RNA containing a methylated blocked 5' terminus by HeLa nuclear homogenates.

Abstract

RNA synthesized in vitro by HeLa nuclei contains a variety of blocked methylated 5'-terminal structures that can be grouped into four general types: (a) m(7)GppN(m)p...; (b) m(7)GppN(m)pM(m)p...; (c) m(7)GpppN(m)p...; and (d) m(7)GpppN(m)pM(m).... Both N(m) and M(m) can be either one of the four 2'-O-methylated nucleosides. The relative amounts of the N(m) species are G = 45%, A = 25%, U = 20%, and C = 10%.

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Year: 1975 PMID： 1059083 PMCID： PMC432892 DOI： 10.1073/pnas.72.8.2930

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

14 in total

1. The methylated constituents of L cell messenger RNA: evidence for an unusual cluster at the 5' terminus.

Authors: R P Perry; D E Kelley; K Friderici; F Rottman
Journal: Cell Date: 1975-04 Impact factor: 41.582

2. Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-.

Authors: Y Furuichi; M Morgan; S Muthukrishnan; A J Shatkin
Journal: Proc Natl Acad Sci U S A Date: 1975-01 Impact factor: 11.205

3. Methylated nucleotides block 5'-terminus of vaccinia virus messenger RNA.

Authors: C M Wei; B Moss
Journal: Proc Natl Acad Sci U S A Date: 1975-01 Impact factor: 11.205

4. Methylation-dependent translation of viral messenger RNAs in vitro.

Authors: G W Both; A K Banerjee; A J Shatkin
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

5. Methylated nucleotides block 5' terminus of HeLa cell messenger RNA.

Authors: C M Wei; A Gershowitz; B Moss
Journal: Cell Date: 1975-04 Impact factor: 41.582

6. Methylated simian virus 40-specific RNA from nuclei and cytoplasm of infected BSC-1 cells.

Authors: S Lavi; A J Shatkin
Journal: Proc Natl Acad Sci U S A Date: 1975-06 Impact factor: 11.205

7. A rapid assay technique for RNA ribose methylases.

Authors: I Svensson; V J Patel
Journal: Nucleic Acids Res Date: 1975-04 Impact factor: 16.971

8. The in vitro synthesis of avian myeloblastosis viral RNA sequences.

Authors: M Jacquet; Y Groner; G Monroy; J Hurwitz
Journal: Proc Natl Acad Sci U S A Date: 1974-08 Impact factor: 11.205

9. Primary sequence of U-1 nuclear ribonucleic acid of Novikoff hepatoma ascites cells.

Authors: R Reddy; T S Ro-Choi; D Henning; H Busch
Journal: J Biol Chem Date: 1974-10-25 Impact factor: 5.157

10. Sequences containing methylated nucleotides at the 5' termini of messenger RNAs: possible implications for processing.

Authors: F Rottman; A J Shatkin; R P Perry
Journal: Cell Date: 1974-11 Impact factor: 41.582

20 in total

8. Initiation of transcription by RNA polymerase II in permeable, SV40-infected or noninfected, CVI cells; evidence for multiple promoters of SV40 late transcription.

Authors: R Contreras; W Fiers
Journal: Nucleic Acids Res Date: 1981-01-24 Impact factor: 16.971

9. Comparative studies on the '5'-cap' and in vitro translational activity of cytoplasmic and nuclear poly A(+) RNA1.

Authors: M S Rao; F Hirsch; B C Wu; W H Spohn; H Busch
Journal: Mol Cell Biochem Date: 1977-03-21 Impact factor: 3.396

10. Intracellular DNA of the parvovirus minute virus of mice is organized in a minichromosome structure.

Authors: E Ben-Asher; S Bratosin; Y Aloni
Journal: J Virol Date: 1982-03 Impact factor: 5.103

Synthesis of RNA containing a methylated blocked 5' terminus by HeLa nuclear homogenates.

1. The methylated constituents of L cell messenger RNA: evidence for an unusual cluster at the 5' terminus.

2. Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-.

3. Methylated nucleotides block 5'-terminus of vaccinia virus messenger RNA.

4. Methylation-dependent translation of viral messenger RNAs in vitro.

5. Methylated nucleotides block 5' terminus of HeLa cell messenger RNA.

6. Methylated simian virus 40-specific RNA from nuclei and cytoplasm of infected BSC-1 cells.

7. A rapid assay technique for RNA ribose methylases.

8. The in vitro synthesis of avian myeloblastosis viral RNA sequences.

9. Primary sequence of U-1 nuclear ribonucleic acid of Novikoff hepatoma ascites cells.

10. Sequences containing methylated nucleotides at the 5' termini of messenger RNAs: possible implications for processing.

1. Identification and mapping of N6-methyladenosine containing sequences in simian virus 40 RNA.

2. Minimal methylation of yeast messenger RNA.

3. Characterization of the major altered leader sequence of late mRNA induced by SV40 deletion mutant d1-1811.

4. Mercurated nucleotides: assessment of a new tool to study RNA synthesis and processing in isolated nuclei.

5. Capping structures of simian virus 40 19S and 16S mRNAs.

6. Transmethylation and transguanylylation in 5'-RNA capping system isolated from rat liver nuclei.

7. Synthesis of messenger RNA-like molecules in isolated myeloma nuclei.

8. Initiation of transcription by RNA polymerase II in permeable, SV40-infected or noninfected, CVI cells; evidence for multiple promoters of SV40 late transcription.

9. Comparative studies on the '5'-cap' and in vitro translational activity of cytoplasmic and nuclear poly A(+) RNA1.

10. Intracellular DNA of the parvovirus minute virus of mice is organized in a minichromosome structure.