Literature DB >> 237257

A rapid assay technique for RNA ribose methylases.

I Svensson, V J Patel.   

Abstract

A rapid technique for quantitative separation of ribose-methylated nucleosides from base-methylated and non-methylated nucleosides by chromatography on DEAE-cellulose paper in the presence of borate is described. The method has been used as an assay for tRNA ribose methylases from yeast, using under methylated Escherichia coli tRNA as substrate. The main product formed with a partly purified yeast enzyme was characterized as 2'-O-methylcytidine.

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Year:  1975        PMID： 237257      PMCID： PMC342867          DOI： 10.1093/nar/2.4.603

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

Review 1.  The tRNA methyltransferases.

Authors:  S J Kerr; E Borek
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1972

2.  An analytical method for the separation of sugar-methylated ribonucleosides from base-methylated and nonmethylated ribonucleosides.

Authors:  A al-Arif; M B Sporn
Journal:  Anal Biochem       Date:  1972-08       Impact factor: 3.365

3.  Gas chromatographic method for determination of 2'-O-methylation in RNA.

Authors:  J Abbate; F Rottman
Journal:  Anal Biochem       Date:  1972-06       Impact factor: 3.365

4.  On the bias of the distribution of the 2'-O-methylribonucleotide constituents of yeast transfer RNA.

Authors:  S Morisawa; E Chargaff
Journal:  Biochim Biophys Acta       Date:  1968-12-17

5.  Aminoacylation and polypeptide synthesis with tRNA lacking ribothymidine.

Authors:  I Svensson; L Isaksson; A Henningsson
Journal:  Biochim Biophys Acta       Date:  1971-05-13

6.  Isolation of mutants of Escherichia coli lac king 5-methyluracil in transfer ribonucleic acid or 1-methylguanine in ribosomal RNA.

Authors:  G R Björk; L A Isaksson
Journal:  J Mol Biol       Date:  1970-07-14       Impact factor: 5.469

7.  A rapid and specific assay for sugar methylation in ribonucleic acid.

Authors:  F Baskin; C A Dekker
Journal:  J Biol Chem       Date:  1967-11-25       Impact factor: 5.157

Review 8.  Methylated ribonucleic acids.

Authors:  J L Starr; B H Sells
Journal:  Physiol Rev       Date:  1969-07       Impact factor: 37.312

9.  The in vitro synthesis of 2'-omethylguanosine and 2-methylthio 6N (gamma,gamma, dimethylallyl) adenosine in transfer RNA of Escherichia coli.

Authors:  M L Gefter
Journal:  Biochem Biophys Res Commun       Date:  1969-08-07       Impact factor: 3.575

10.  N-4-methyl-2'-O-methyl cytidine and other methyl-substituted nucleoside constituents of Escherichia coli ribosomal and soluble RNA.

Authors:  J L Nichols; B G Lane
Journal:  Biochim Biophys Acta       Date:  1966-06-22
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  2 in total

1.  Synthesis of RNA containing a methylated blocked 5' terminus by HeLa nuclear homogenates.

Authors:  Y Groner; J Hurwitz
Journal:  Proc Natl Acad Sci U S A       Date:  1975-08       Impact factor: 11.205

2.  Modified nucleosides of Bacillus subtilis transfer ribonucleic acids.

Authors:  B Vold
Journal:  J Bacteriol       Date:  1976-07       Impact factor: 3.490
  2 in total

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