Literature DB >> 10590102

Ultrastructural and functional analyses of recombinant influenza virus ribonucleoproteins suggest dimerization of nucleoprotein during virus amplification.

J Ortega1, J Martín-Benito, T Zürcher, J M Valpuesta, J L Carrascosa, J Ortín.   

Abstract

Influenza virus ribonucleoproteins (RNPs) were reconstituted in vivo from cloned cDNAs expressing the three polymerase subunits, the nucleoprotein (NP), and short template RNAs. The structure of purified RNPs was studied by electron microscopy and image processing. Circular and elliptic structures were obtained in which the NP and the polymerase complex could be defined. Comparison of the structure of RNPs of various lengths indicated that each NP monomer interacts with approximately 24 nucleotides. The analysis of the amplification of RNPs with different lengths showed that those with the highest replication efficiency contained an even number of NP monomers, suggesting that the NP is incorporated as dimers into newly synthesized RNPs.

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Year:  2000        PMID： 10590102      PMCID： PMC111524          DOI： 10.1128/jvi.74.1.156-163.2000

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  72 in total

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2.  A new resolution criterion based on spectral signal-to-noise ratios.

Authors:  M Unser; B L Trus; A C Steven
Journal:  Ultramicroscopy       Date:  1987       Impact factor: 2.689

3.  Characterization of influenza virus RNA complete transcripts.

Authors:  A J Hay; J J Skehel; J McCauley
Journal:  Virology       Date:  1982-01-30       Impact factor: 3.616

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Authors:  M H Heggeness; P R Smith; I Ulmanen; R M Krug; P W Choppin
Journal:  Virology       Date:  1982-04-30       Impact factor: 3.616

5.  Identification of the cap binding protein of influenza virus.

Authors:  D Blaas; E Patzelt; E Kuechler
Journal:  Nucleic Acids Res       Date:  1982-08-11       Impact factor: 16.971

6.  Use of a hybrid vaccinia virus-T7 RNA polymerase system for expression of target genes.

Authors:  T R Fuerst; P L Earl; B Moss
Journal:  Mol Cell Biol       Date:  1987-07       Impact factor: 4.272

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Authors:  I Ulmanen; B A Broni; R M Krug
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

8.  Does the higher order structure of the influenza virus ribonucleoprotein guide sequence rearrangements in influenza viral RNA?

Authors:  P A Jennings; J T Finch; G Winter; J S Robertson
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9.  Assembly of influenza ribonucleoprotein in vitro using recombinant nucleoprotein.

Authors:  D W Kingsbury; I M Jones; K G Murti
Journal:  Virology       Date:  1987-02       Impact factor: 3.616

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Authors:  A Kato; K Mizumoto; A Ishihama
Journal:  Virus Res       Date:  1985-09       Impact factor: 3.303
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  56 in total

1.  Three-dimensional reconstruction of a recombinant influenza virus ribonucleoprotein particle.

Authors:  J Martín-Benito; E Area; J Ortega; O Llorca; J M Valpuesta; J L Carrascosa; J Ortín
Journal:  EMBO Rep       Date:  2001-04       Impact factor: 8.807

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Journal:  J Virol       Date:  2001-08       Impact factor: 5.103

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Journal:  J Biol Chem       Date:  2010-06-10       Impact factor: 5.157

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Journal:  Proc Natl Acad Sci U S A       Date:  2003-12-22       Impact factor: 11.205

5.  Mutations in the N-terminal region of influenza virus PB2 protein affect virus RNA replication but not transcription.

Authors:  Pablo Gastaminza; Beatriz Perales; Ana M Falcón; Juan Ortín
Journal:  J Virol       Date:  2003-05       Impact factor: 5.103

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7.  E339...R416 salt bridge of nucleoprotein as a feasible target for influenza virus inhibitors.

Authors:  Yu-Fang Shen; Yu-Hou Chen; Shao-Ying Chu; Meng-I Lin; Hua-Ting Hsu; Pei-Yu Wu; Chao-Jung Wu; Hui-Wen Liu; Fu-Yang Lin; Gialih Lin; Pang-Hung Hsu; An-Suei Yang; Yih-Shyun E Cheng; Ying-Ta Wu; Chi-Huey Wong; Ming-Daw Tsai
Journal:  Proc Natl Acad Sci U S A       Date:  2011-09-19       Impact factor: 11.205

8.  Architecture and regulation of negative-strand viral enzymatic machinery.

Authors:  Philip J Kranzusch; Sean P J Whelan
Journal:  RNA Biol       Date:  2012-07-01       Impact factor: 4.652

9.  Genetic analysis of influenza virus NS1 gene: a temperature-sensitive mutant shows defective formation of virus particles.

Authors:  Urtzi Garaigorta; Ana M Falcón; Juan Ortín
Journal:  J Virol       Date:  2005-12       Impact factor: 5.103

10.  Genetic trans-complementation establishes a new model for influenza virus RNA transcription and replication.

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Journal:  PLoS Pathog       Date:  2009-05-29       Impact factor: 6.823
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