Literature DB >> 14691253

3D structure of the influenza virus polymerase complex: localization of subunit domains.

Estela Area1, Jaime Martín-Benito, Pablo Gastaminza, Eva Torreira, José M Valpuesta, José L Carrascosa, Juan Ortín.   

Abstract

The 3D structure of the influenza virus polymerase complex was determined by electron microscopy and image processing of recombinant ribonucleoproteins (RNPs). The RNPs were generated by in vivo amplification using cDNAs of the three polymerase subunits, the nucleoprotein, and a model virus-associated RNA containing 248 nt. The polymerase structure obtained is very compact, with no apparent boundaries among subunits. The position of specific regions of the PB1, PB2, and PA subunits was determined by 3D reconstruction of either RNP-mAb complexes or tagged RNPs. This structural model is available for the polymerase of a negative-stranded RNA virus and provides a general delineation of the complex and its interaction with the template-associated nucleoprotein monomers in the RNP.

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Year:  2003        PMID： 14691253      PMCID： PMC314181          DOI： 10.1073/pnas.0307127101

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


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