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Literature DB >> 105726

An improved assay technique for uridine diphosphate glucuronosyltransferase activity towards 5-hydroxytryptamine and some properties of the enzyme.

Abstract

A simplified and sensitive procedure for the routine assay of UDP-glucuronosyltransferase activity towards 5-hydroxytryptamine (serotonin) was developed and the reaction product confirmed as the O-glucuronide of this substrate. The assay was used to study some properties of this UDP-glucuronosyltransferase activity. In mouse liver activity was stimulated by membrane-perturbation procedures and by UDP-N-acetylglucosamine. In rat liver it was stimulated by digitonin, but not by diethylnitrosamine. Mouse duodenum, kidney, and lung possessed activity that was less latent than in liver. No activity was found in homogenates of brain. The activity was present in Gunn rat liver, though only one-third of that in Wistar rat liver. Cat liver contained no UDP-glucuronosyltransferase activity towards 5-hydroxytryptamine.

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Year: 1978 PMID： 105726 PMCID： PMC1186175 DOI： 10.1042/bj1751119

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

20 in total

1. Functional heterogeneity of UDP-glucuronosyltransferase as indicated by its differential development and inducibility by glucocorticoids. Demonstration of two groups within the enzyme's activity towards twelve substrates.

Authors: G J Wishart
Journal: Biochem J Date: 1978-08-15 Impact factor: 3.857

2. In vivo metabolism of serotonin and tryptamine: effect of monoamine oxidase inhibition.

Authors: H WEISSBACH; W LOVENBERG; B G REDFIELD; S UDENFRIEND
Journal: J Pharmacol Exp Ther Date: 1961-01 Impact factor: 4.030

3. Nucleotide activation of liver microsomal glucuronidation.

Authors: B M POGELL; L F LELOIR
Journal: J Biol Chem Date: 1961-02 Impact factor: 5.157

4. Congenital jaundice in rats, due to a defect in glucuronide formation.

Authors: R SCHMID; J AXELROD; L HAMMAKER; R L SWARM
Journal: J Clin Invest Date: 1958-08 Impact factor: 14.808

5. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

6. Metabolism of serotonin by the isolated perfused rat liver--effect of glucuronyl transferase deficiency or monoamine oxidase inhibition.

Authors: G M Tyce; E V Flock; C A Owen
Journal: Biochem Pharmacol Date: 1968-08 Impact factor: 5.858

7. Kinetic properties of microsomal UDP-glucuronyltransferase. Evidence for cooperative kinetics and activation by UDP-N-acetylglucosamine.

Authors: D A Vessey; J Goldenberg; D Zakim
Journal: Biochim Biophys Acta Date: 1973-05-05

8. Spontaneous and detergent activation of a glucuronyltransferase in vitro.

Authors: K K Lueders; E L Kuff
Journal: Arch Biochem Biophys Date: 1967-04 Impact factor: 4.013

9. Studies on the purification of rat liver uridine diphosphate glucuronyltransferase.

Authors: B Burchell
Journal: Biochem J Date: 1977-03-01 Impact factor: 3.857

10. Precocious development of UDP-glucuronyltransferase activity in chick-embryo liver after administration of adrenocorticotropic hormone and of certain 11beta-hydroxy corticosteroids.

Authors: J E Leakey; G J Wishart; G J Dutton
Journal: Biochem J Date: 1976-08-15 Impact factor: 3.857

4 in total

1. Immunochemical analysis of uridine diphosphate-glucuronosyltransferase in four patients with the Crigler-Najjar syndrome type I.

Authors: H H van Es; B G Goldhoorn; M Paul-Abrahamse; R P Elferink; P L Jansen
Journal: J Clin Invest Date: 1990-04 Impact factor: 14.808