Literature DB >> 10570794

Stimulatory effect of growth in the presence of alcohols on biotransformation of penicillin G into cephalosporin-type antibiotics by resting cells of Streptomyces clavuligerus NP1.

M J Fernández1, J L Adrio, J M Piret, S Wolfe, S Ro, A L Demain.   

Abstract

Growth of Streptomyces clavuligerus NP1 in the presence of methanol or ethanol resulted in a marked increase in production of cephalosporin(s) from penicillin G by resting cells. The mycelium produced in alcohol-supplemented medium was fragmented and dispersed as compared with growth in control medium. HPLC analysis showed that at least two products were present in the biotransformation supernatant fluid after 1 h incubation. One of them has been identified as deacetoxycephalosporin G (DAOG).

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Year:  1999        PMID： 10570794     DOI： 10.1007/s002530051549

Source DB:  PubMed          Journal:  Appl Microbiol Biotechnol        ISSN： 0175-7598            Impact factor:   4.813


  5 in total

1.  Engineering Streptomyces clavuligerus deacetoxycephalosporin C synthase for optimal ring expansion activity toward penicillin G.

Authors:  Chia-Li Wei; Yunn-Bor Yang; Wen-Ching Wang; Wen-Chi Liu; Jyh-Shing Hsu; Ying-Chieh Tsai
Journal:  Appl Environ Microbiol       Date:  2003-04       Impact factor: 4.792

2.  Performance of a recombinant strain of Streptomyces lividans for bioconversion of penicillin G to deacetoxycephalosporin G.

Authors:  Q Gao; J M Piret; J L Adrio; A L Demain
Journal:  J Ind Microbiol Biotechnol       Date:  2003-03-14       Impact factor: 3.346

Review 3.  Directed evolution and rational approaches to improving Streptomyces clavuligerus deacetoxycephalosporin C synthase for cephalosporin production.

Authors:  Kian-Sim Goo; Chun-Song Chua; Tiow-Suan Sim
Journal:  J Ind Microbiol Biotechnol       Date:  2009-03-07       Impact factor: 3.346

Review 4.  Engineering deacetoxycephalosporin C synthase as a catalyst for the bioconversion of penicillins.

Authors:  Keqiang Fan; Baixue Lin; Yong Tao; Keqian Yang
Journal:  J Ind Microbiol Biotechnol       Date:  2016-11-08       Impact factor: 3.346

5.  Reconstitution of TCA cycle with DAOCS to engineer Escherichia coli into an efficient whole cell catalyst of penicillin G.

Authors:  Baixue Lin; Keqiang Fan; Jian Zhao; Junjie Ji; Linjun Wu; Keqian Yang; Yong Tao
Journal:  Proc Natl Acad Sci U S A       Date:  2015-07-27       Impact factor: 11.205
  5 in total

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