Literature DB >> 12715257

Performance of a recombinant strain of Streptomyces lividans for bioconversion of penicillin G to deacetoxycephalosporin G.

Q Gao1, J M Piret, J L Adrio, A L Demain.   

Abstract

We examined the performance of Streptomyces lividans strain W25 containing a hybrid expandase (deacetoxycephalosporin C synthase; DAOCS) gene, obtained by in vivo recombination between the expandase genes of S. clavuligerus and Nocardia lactamdurans for resting-cell bioconversion of penicillin G to deacetoxycephalosporin G. Strain W25 carried out a much more effective level of bioconversion than the previously used strain, S. clavuligerus NP1. The two strains also differed in the concentrations of FeSO(4) and alpha-ketoglutarate giving maximal activity. Whereas NP1 preferred 1.8 mM FeSO(4 )and 1.3 mM alpha-ketoglutarate, recombinant W25 performed best at 0.45 mM FeSO(4) and 1.9 mM alpha-ketoglutarate.

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Year:  2003        PMID： 12715257     DOI： 10.1007/s10295-003-0034-4

Source DB:  PubMed          Journal:  J Ind Microbiol Biotechnol        ISSN： 1367-5435            Impact factor:   3.346


  11 in total

1.  Elucidation of conditions allowing conversion of penicillin G and other penicillins to deacetoxycephalosporins by resting cells and extracts of Streptomyces clavuligerus NP1.

Authors:  H Cho; J L Adrio; J M Luengo; S Wolfe; S Ocran; G Hintermann; J M Piret; A L Demain
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-29       Impact factor: 11.205

2.  Conversion of penicillin N to cephalosporin(s) by cell-free extracts of Cephalosporium acremonium.

Authors:  M Kohsaka; A L Demain
Journal:  Biochem Biophys Res Commun       Date:  1976-05-17       Impact factor: 3.575

3.  Effect of solvents on bioconversion of penicillin G to deacetoxycephalosporin G.

Authors:  Q Gao; A L Demain
Journal:  J Antibiot (Tokyo)       Date:  2001-11       Impact factor: 2.649

4.  Stimulatory effect of growth in the presence of alcohols on biotransformation of penicillin G into cephalosporin-type antibiotics by resting cells of Streptomyces clavuligerus NP1.

Authors:  M J Fernández; J L Adrio; J M Piret; S Wolfe; S Ro; A L Demain
Journal:  Appl Microbiol Biotechnol       Date:  1999-10       Impact factor: 4.813

5.  Chemistry of cephalosporin antibiotics. XXI. Conversion of penicillins to cephalexin.

Authors:  R R Chauvette; P A Pennington; C W Ryan; R D Cooper; F L José; I G Wright; E M Van Heyningen; G W Huffman
Journal:  J Org Chem       Date:  1971-05-07       Impact factor: 4.354

6.  The nature of the stimulation of the growth of Streptococcus lactis by yeast extract.

Authors:  J S Smith; A J Hillier; G J Lees
Journal:  J Dairy Res       Date:  1975-02       Impact factor: 1.904

7.  Immobilized Streptomyces clavuligerus NP1 cells for biotransformation of penicillin G into deacetoxycephalosporin G.

Authors:  A L Demain; M A Báez-Vásquez
Journal:  Appl Biochem Biotechnol       Date:  2000-05       Impact factor: 2.926

8.  Improvement in the resting-cell bioconversion of penicillin G to deacetoxycephalosporin G by addition of catalase.

Authors:  Q Gao; A L Demain
Journal:  Lett Appl Microbiol       Date:  2002       Impact factor: 2.858

9.  Improvement in the bioconversion of penicillin G to deacetoxycephalosporin G by elimination of agitation and addition of decane.

Authors:  Q Gao; A L Demain
Journal:  Appl Microbiol Biotechnol       Date:  2001-11       Impact factor: 4.813

10.  Cephalosporin formation by cell-free extracts from Streptomyces clavuligerus.

Authors:  S E Jensen; D W Westlake; R J Bowers; S Wolfe
Journal:  J Antibiot (Tokyo)       Date:  1982-10       Impact factor: 2.649
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  6 in total

1.  Relevant double mutations in bioengineered Streptomyces clavuligerus deacetoxycephalosporin C synthase result in higher binding specificities which improve penicillin bioconversion.

Authors:  Kian Sim Goo; Chun Song Chua; Tiow-Suan Sim
Journal:  Appl Environ Microbiol       Date:  2007-12-14       Impact factor: 4.792

Review 2.  Directed evolution and rational approaches to improving Streptomyces clavuligerus deacetoxycephalosporin C synthase for cephalosporin production.

Authors:  Kian-Sim Goo; Chun-Song Chua; Tiow-Suan Sim
Journal:  J Ind Microbiol Biotechnol       Date:  2009-03-07       Impact factor: 3.346

Review 3.  Engineering deacetoxycephalosporin C synthase as a catalyst for the bioconversion of penicillins.

Authors:  Keqiang Fan; Baixue Lin; Yong Tao; Keqian Yang
Journal:  J Ind Microbiol Biotechnol       Date:  2016-11-08       Impact factor: 3.346

4.  Reconstitution of TCA cycle with DAOCS to engineer Escherichia coli into an efficient whole cell catalyst of penicillin G.

Authors:  Baixue Lin; Keqiang Fan; Jian Zhao; Junjie Ji; Linjun Wu; Keqian Yang; Yong Tao
Journal:  Proc Natl Acad Sci U S A       Date:  2015-07-27       Impact factor: 11.205

5.  Deacetoxycephalosporin C synthase (expandase): Research progress and application potential.

Authors:  Xiaofan Niu; Jian Zhang; Xianli Xue; Depei Wang; Lin Wang; Qiang Gao
Journal:  Synth Syst Biotechnol       Date:  2021-11-23

6.  Using RNA-Sequencing Data to Examine Tissue-Specific Garlic Microbiomes.

Authors:  Yeonhwa Jo; Chang-Gi Back; Kook-Hyung Kim; Hyosub Chu; Jeong Hun Lee; Sang Hyun Moh; Won Kyong Cho
Journal:  Int J Mol Sci       Date:  2021-06-24       Impact factor: 5.923
  6 in total

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