| Literature DB >> 10549298 |
K Natarajan1, B M Jackson, H Zhou, F Winston, A G Hinnebusch.
Abstract
Mutations in three subunits of the SWI/SNF complex and in the Med2p subunit of the SRB/mediator of pol II holoenzyme impaired Gcn4p-activated transcription of HIS3 without reducing Gcn4p-independent transcription of this gene. Recombinant Gcn4p interacted with SWI/SNF and SRB/mediator subunits in cell extracts in a manner dependent on the same hydrophobic clusters in the Gcn4p activation domain; however, higher concentrations of Gcn4p were required for binding to SWI/SNF versus SRB/mediator subunits. In addition, SRB/mediator and SWI/SNF subunits did not coimmunopreciptate from the extracts. These findings, together with the fact that Gcn4p specifically interacted with purified SWI/SNF, strongly suggest that Gcn4p independently recruits SWI/SNF and holoenzyme to its target promoters in the course of activating transcription.Entities:
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Year: 1999 PMID： 10549298 DOI： 10.1016/s1097-2765(00)80217-8
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970