| Literature DB >> 10545992 |
F S Santiago1, H C Lowe, M M Kavurma, C N Chesterman, A Baker, D G Atkins, L M Khachigian.
Abstract
Early growth response factor-1 (Egr-1) binds to the promoters of many genes whose products influence cell movement and replication in the artery wall. Here we targeted Egr-1 using a new class of DNA-based enzyme that specifically cleaved Egr-1 mRNA, blocked induction of Egr-1 protein, and inhibited cell proliferation and wound repair in culture. The DNA enzyme also inhibited Egr-1 induction and neointima formation after balloon injury to the rat carotid artery wall. These findings demonstrate the utility of DNA enzymes as biological tools to delineate the specific functions of a given gene, and implicate catalytic nucleic acid molecules composed entirely of DNA as potential therapeutic agents.Entities:
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Year: 1999 PMID： 10545992 DOI： 10.1038/15215
Source DB: PubMed Journal: Nat Med ISSN： 1078-8956 Impact factor: 53.440