Literature DB >> 11825996

Rapid real-time PCR for determination of penicillin susceptibility in pneumococcal meningitis, including culture-negative cases.

Angela M Kearns1, Clive Graham, Doreen Burdess, Jane Heatherington, Roger Freeman.   

Abstract

A novel real-time PCR-hybridization assay was developed for the rapid (<1 h) detection of penicillin susceptibility in Streptococcus pneumoniae. When applied to 24 pneumococcal DNA-positive cerebrospinal fluid extracts, penicillin-sensitive S. pneumoniae was detected in all instances. Real-time PCR proved more sensitive than culture, microscopy, or antigen detection and provided susceptibility data even in culture-negative cases.

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Year:  2002        PMID： 11825996      PMCID： PMC153399          DOI： 10.1128/JCM.40.2.682-684.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

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4.  Determination of penicillin susceptibility of Streptococcus pneumoniae using the polymerase chain reaction.

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5.  Combinational detection of autolysin and penicillin-binding protein 2B genes of Streptococcus pneumoniae by PCR.

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8.  Bacterial meningitis in the United States in 1995. Active Surveillance Team.

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9.  Evolution of penicillin resistance in Streptococcus pneumoniae; the role of Streptococcus mitis in the formation of a low affinity PBP2B in S. pneumoniae.

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Journal:  Mol Microbiol       Date:  1993-08       Impact factor: 3.501

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Authors:  D J Waghorn
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4.  Serial PCR genetic load determination in the surgical management of pneumococcal intracranial sepsis.

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8.  Clinical features, aetiology and outcome of empyema in children in the north east of England.

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Review 9.  Biological and Epidemiological Features of Antibiotic-Resistant Streptococcus pneumoniae in Pre- and Post-Conjugate Vaccine Eras: a United States Perspective.

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Review 10.  The modern autopsy: what to do if infection is suspected.

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