Literature DB >> 10518927

A 6 bp Z-DNA hairpin binds two Z alpha domains from the human RNA editing enzyme ADAR1.

M Schade1, J Behlke, K Lowenhaupt, A Herbert, A Rich, H Oschkinat.   

Abstract

The Z alpha domain of the human RNA editing enzyme double-stranded RNA deaminase I (ADAR1) binds to left-handed Z-DNA with high affinity. We found by analytical ultracentrifugation and CD spectroscopy that two Z alpha domains bind to one d(CG)3T4(CG)3 hairpin which contains a stem of six base pairs in the Z-DNA conformation. Both wild-type Z alpha and a C125S mutant show a mean dissociation constant of 30 nM as measured by surface plasmon resonance and analytical ultracentrifugation. Our data suggest that short (> or = 6 bp) segments of Z-DNA within a gene are able to recruit two ADAR1 enzymes to that particular site.

Entities:  

Keywords:  Non-programmatic

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Year:  1999        PMID： 10518927     DOI： 10.1016/s0014-5793(99)01119-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  8 in total

1.  The solution structure of the Zalpha domain of the human RNA editing enzyme ADAR1 reveals a prepositioned binding surface for Z-DNA.

Authors:  M Schade; C J Turner; R Kühne; P Schmieder; K Lowenhaupt; A Herbert; A Rich; H Oschkinat
Journal:  Proc Natl Acad Sci U S A       Date:  1999-10-26       Impact factor: 11.205

2.  The zalpha domain of the editing enzyme dsRNA adenosine deaminase binds left-handed Z-RNA as well as Z-DNA.

Authors:  B A Brown; K Lowenhaupt; C M Wilbert; E B Hanlon; A Rich
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-05       Impact factor: 11.205

Review 3.  Sedimentation equilibrium: a valuable tool to study homologous and heterogeneous interactions of proteins or proteins and nucleic acids.

Authors:  Joachim Behlke; Otto Ristau
Journal:  Eur Biophys J       Date:  2003-05-29       Impact factor: 1.733

4.  Alternate rRNA secondary structures as regulators of translation.

Authors:  Shu Feng; Heng Li; Jing Zhao; Konstantin Pervushin; Ky Lowenhaupt; Thomas U Schwartz; Peter Dröge
Journal:  Nat Struct Mol Biol       Date:  2011-01-09       Impact factor: 15.369

5.  The solution structure of the N-terminal domain of E3L shows a tyrosine conformation that may explain its reduced affinity to Z-DNA in vitro.

Authors:  Jan D Kahmann; Diana A Wecking; Vera Putter; Ky Lowenhaupt; Yang-Gyun Kim; Peter Schmieder; Hartmut Oschkinat; Alexander Rich; Markus Schade
Journal:  Proc Natl Acad Sci U S A       Date:  2004-02-23       Impact factor: 11.205

Review 6.  The dynamic epitranscriptome: A to I editing modulates genetic information.

Authors:  Mansoureh Tajaddod; Michael F Jantsch; Konstantin Licht
Journal:  Chromosoma       Date:  2015-07-07       Impact factor: 4.316

Review 7.  Z-DNA and Z-RNA in human disease.

Authors:  Alan Herbert
Journal:  Commun Biol       Date:  2019-01-07

Review 8.  The Role of the Z-DNA Binding Domain in Innate Immunity and Stress Granules.

Authors:  De Chen Chiang; Yan Li; Siew Kit Ng
Journal:  Front Immunol       Date:  2021-02-03       Impact factor: 7.561
  8 in total

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