| Literature DB >> 10514983 |
A Yanagida1, T Kanda, T Shoji, M OhnishiKameyama, T Nagata.
Abstract
Oligomeric constituents of apple procyanidins were fractionated by size-exclusion chromatography using a TSKgel Toyopearl HW-40F column. The best separation was obtained using a mobile phase of acetone-8 M urea (6:4; adjusted to pH 2) at a flow-rate of 1.0 ml/min. In this chromatographic system, the use of 8 M urea in the mobile phase resulted in a molecular sieve effect without any surface affinity interaction between the gel beads and the procyanidin molecules. Each fraction obtained was examined by reversed-phase high-performance liquid chromatography and time-of-flight mass spectrometry. The order of elution of the procyanidins from the column was coincident with their degree of polymerization.Entities:
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Year: 1999 PMID： 10514983 DOI： 10.1016/s0021-9673(99)00684-6
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759