SETTING: Peptidenucleic acid (PNA) probesdesigned for specific detection of mycobacteria of the Mycobacterium tuberculosis complex (MTC) and other non-tuberculous mycobacterium species (NTM) are shown to be able to penetrate the mycobacterial cell wall and subsequently hybridize in situ to complementary rRNA. OBJECTIVE: To demonstrate the use of fluorescein-labelled PNA probes for detection and identification of M. tuberculosis in smear-positive sputum samples. DESIGN: The sensitivity and specificity of the PNA probes were investigated by fluorescence in situ hybridization (FISH) using cultures of mycobacterium strains representing species of the MTC and NTM, respectively. RESULTS: M. tuberculosis strains were detected by FISH using specific fluorescein-labelled PNA probes directly in smear-positive sputum samples without changing the morphology of the cells. CONCLUSION: PNA probes allow for rapid diagnosis of tuberculosis in smear-positive cases.
SETTING:Peptidenucleic acid (PNA) probesdesigned for specific detection of mycobacteria of the Mycobacterium tuberculosis complex (MTC) and other non-tuberculous mycobacterium species (NTM) are shown to be able to penetrate the mycobacterial cell wall and subsequently hybridize in situ to complementary rRNA. OBJECTIVE: To demonstrate the use of fluorescein-labelled PNA probes for detection and identification of M. tuberculosis in smear-positive sputum samples. DESIGN: The sensitivity and specificity of the PNA probes were investigated by fluorescence in situ hybridization (FISH) using cultures of mycobacterium strains representing species of the MTC and NTM, respectively. RESULTS:M. tuberculosis strains were detected by FISH using specific fluorescein-labelled PNA probes directly in smear-positive sputum samples without changing the morphology of the cells. CONCLUSION:PNA probes allow for rapid diagnosis of tuberculosis in smear-positive cases.
Authors: H Stender; C Kurtzman; J J Hyldig-Nielsen; D Sørensen; A Broomer; K Oliveira; H Perry-O'Keefe; A Sage; B Young; J Coull Journal: Appl Environ Microbiol Date: 2001-02 Impact factor: 4.792
Authors: Susan Rigby; Gary W Procop; Gerhard Haase; Deborah Wilson; Geraldine Hall; Cletus Kurtzman; Kenneth Oliveira; Sabina Von Oy; Jens J Hyldig-Nielsen; James Coull; Henrik Stender Journal: J Clin Microbiol Date: 2002-06 Impact factor: 5.948
Authors: V González; E Padilla; M Giménez; C Vilaplana; A Pérez; G Fernández; M D Quesada; M A Pallarés; V Ausina Journal: Eur J Clin Microbiol Infect Dis Date: 2004-04-27 Impact factor: 3.267
Authors: Laura Cerqueira; Nuno F Azevedo; Carina Almeida; Tatiana Jardim; Charles William Keevil; Maria J Vieira Journal: Int J Mol Sci Date: 2008-10-20 Impact factor: 5.923