Literature DB >> 104714

The immobilization of adenine nucleotides on polysaccharides by using glutaraldehyde coupling and borohydride reduction.

P Gacesa, W J Whish.   

Abstract

Adenine nucleotides were immobilized on modified Sepharose 4B or Dextran T40 with glutaraldehyde and reduced with KBH4. Binding was dependent on pH and the nature of the amino group on the modified polysaccharide. ATP bound to soluble dextran retained coenzyme activity with glycerol kinase. Binding is proposed to occur via a Schiff base.

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Year:  1978        PMID： 104714      PMCID： PMC1186073          DOI： 10.1042/bj1750349

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  11 in total

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Authors:  C W Fuller; H J Bright
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5.  Continuous regeneration of NAD(P)+ by flavins covalently bound to sepharose.

Authors:  M O Månsson; B Mattiasson; S Gestrelius; K Mosbach
Journal:  Biotechnol Bioeng       Date:  1976-08       Impact factor: 4.530

6.  A new immobilized NAD+ analogue, its application in affinity chromatography and as a functioning coenzyme.

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9.  A facile method for the preparation of N6-substituted ATP-Sepharose.

Authors:  F Eckstein; M Goumet; R Wetzel
Journal:  Nucleic Acids Res       Date:  1975-10       Impact factor: 16.971

10.  Affinity chromatography on immobilised adenosine 5'-monophosphate. 1. A new synthesis and some properties of an N6-immobilised 5'-AMP.

Authors:  D B Craven; M J Harvey; C R Lowe; P D Dean
Journal:  Eur J Biochem       Date:  1974-01-16
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  3 in total

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Authors:  P Gacesa; R F Venn
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