Literature DB >> 4147413

The purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors.

C R Lowe, M J Harvey, D B Craven, M A Kerfoot, M E Hollows, P D Dean.   

Abstract

1. The general applicability of affinity chromatography to the purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors is illustrated with several examples taken from crude systems. 2. Methods for overcoming the inevitable loss of selectivity experienced with these polymers are suggested. Effective use of the appropriate nucleotide, the second substrate and other interacting ligands can be made to selectively alter the chromatographic behaviour of the desired enzyme.

Entities:  

Mesh：

Substances：

Year:  1973        PMID： 4147413      PMCID： PMC1177728          DOI： 10.1042/bj1330507

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  19 in total

1.  Affinity chromatography of enzymes on insolubilized cofactors.

Authors:  C R. Lowe; P D.G. Dean
Journal:  FEBS Lett       Date:  1971-05-20       Impact factor: 4.124

2.  Affinity chromatography of lactate dehydrogenase Model studies demonstrating the potential of the technique in the mechanistic investigation as well as in the purification of multi-substrate enzymes.

Authors:  P O'Carra; S Barry
Journal:  FEBS Lett       Date:  1972-04-01       Impact factor: 4.124

3.  Some parameters relevant to affinity chromatography on immobilized nucleotides.

Authors:  C R Lowe; M J Harvey; D B Craven; P D Dean
Journal:  Biochem J       Date:  1973-07       Impact factor: 3.857

4.  Preparation of a NAD(H)-polymer matrix showing coenzyme function of the bound pyridine nucleotide.

Authors:  P O Larsson; K Mosbach
Journal:  Biotechnol Bioeng       Date:  1971-05       Impact factor: 4.530

Review 5.  Affinity chromatography.

Authors:  P Cuatrecasas
Journal:  Annu Rev Biochem       Date:  1971       Impact factor: 23.643

6.  Purification of tetrahydrofolate dehydrogenase by affinity chromatography.

Authors:  J Gauldie; B L Hillcoat
Journal:  Biochim Biophys Acta       Date:  1972-04-07

7.  Some applications of insolubilised cofactors to the purification of pyridine nucleotide-dependent dehydrogenases.

Authors:  C R Lowe; K Mosbach; P D Dean
Journal:  Biochem Biophys Res Commun       Date:  1972-08-21       Impact factor: 3.575

8.  Threonine metabolism via two-carbon compounds by Pseudomonas oxalaticus.

Authors:  M A Blackmore; J M Turner
Journal:  J Gen Microbiol       Date:  1971-08

9.  Affinity chromatography of transaminases.

Authors:  R Collier; G Kohlhaw
Journal:  Anal Biochem       Date:  1971-07       Impact factor: 3.365

10.  Purification and properties of crystalline 3-hydroxybutyrate dehydrogenase from Rhodopseudomonas spheroides.

Authors:  H U Bergmeyer; K Gawehn; H Klotzsch; H A Krebs; D H Williamson
Journal:  Biochem J       Date:  1967-02       Impact factor: 3.857
View more
  4 in total

1.  Affinity chromatography of nicotinamide nucleotide-dependent dehydrogenases on immobilized nucleotide derivatives.

Authors:  I P Trayer; H R Trayer
Journal:  Biochem J       Date:  1974-09       Impact factor: 3.857

2.  An examination of potential matrices for the affinity chromatography of NADP+-linked dehydrogenases with special reference to 6-phosphogluconate dehydrogenase.

Authors:  P Griffiths; A Houlton; M J Adams; M J Harvey; P D Dean
Journal:  Biochem J       Date:  1977-03-01       Impact factor: 3.857

3.  The immobilization of adenine nucleotides on polysaccharides by using glutaraldehyde coupling and borohydride reduction.

Authors:  P Gacesa; W J Whish
Journal:  Biochem J       Date:  1978-10-01       Impact factor: 3.857

4.  Preparation of adenosine nucleotide derivatives suitable for affinity chromatography.

Authors:  I P Trayer; H R Trayer; D P Small; R C Bottomley
Journal:  Biochem J       Date:  1974-06       Impact factor: 3.857
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.