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Literature DB >> 10468554

Chip-based genotyping by mass spectrometry.

K Tang¹, D J Fu, D Julien, A Braun, C R Cantor, H Köster.

Abstract

Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic DNAs were amplified with PCR, and the amplified products were covalently attached to chip wells via N-succinimidyl (4-iodoacetyl)aminobenzoate (SIAB) chemistry. Primer annealing, extension, and termination were performed on a 1-microl scale directly in the chip wells in parallel. Diagnostic products thus generated were detected in situ by using matrix-assisted laser desorption ionization mass spectrometry. This miniaturized method has the potential for accurate, high-throughput, low-cost identification of genetic variations.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1999 PMID： 10468554 PMCID： PMC17834 DOI： 10.1073/pnas.96.18.10016

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

16 in total

Chip-based genotyping by mass spectrometry.

Review 1. The molecular immunology of human platelet proteins.

2. Accessing genetic information with high-density DNA arrays.

3. A strategy for rapid and efficient DNA sequencing by mass spectrometry.

4. Improved analysis of microsatellites using mass spectrometry.

Review 5. Mass spectrometry from miniaturized arrays for full comparative DNA analysis.

6. Sequencing exons 5 to 8 of the p53 gene by MALDI-TOF mass spectrometry.

7. Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes.

8. Matrix-assisted laser desorption/ionization of restriction enzyme-digested DNA.

9. New goals for the U.S. Human Genome Project: 1998-2003.

10. Rapid determination of platelet alloantigen genotypes by polymerase chain reaction using allele-specific primers.

1. Multiplex allele-specific target amplification based on PCR suppression.

2. Homogeneous assays for single-nucleotide polymorphism typing using AlphaScreen.

3. PCR candidate region mismatch scanning: adaptation to quantitative, high-throughput genotyping.

4. Full flexibility genotyping of single nucleotide polymorphisms by the GOOD assay.

5. A system for specific, high-throughput genotyping by allele-specific primer extension on microarrays.

6. A new MALDI-TOF based mini-sequencing assay for genotyping of SNPS.

7. Facile method for automated genotyping of single nucleotide polymorphisms by mass spectrometry.

8. MALDI mass spectrometry analysis of single nucleotide polymorphisms by photocleavage and charge-tagging.

9. Molecular haplotyping at high throughput.

10. Single nucleotide polymorphism seeking long term association with complex disease.