Literature DB >> 10464070

Reporter gene assay for fish-killing activity produced by Pfiesteria piscicida.

E R Fairey1, J S Edmunds, N J Deamer-Melia, H Glasgow, F M Johnson, P R Moeller, J M Burkholder, J S Ramsdell.   

Abstract

Collaborative studies were performed to develop a functional assay for fish-killing activity produced by Pfiesteria piscicida. Eight cell lines were used to screen organic fractions and residual water fraction by using a 3-[4, 5-dimethylthiazol-(2-4)]-diphenyltetrazolium bromide cytotoxicity assay. Diethyl ether and a residual water fraction were cytotoxic to several cell lines including rat pituitary (GH(4)C(1)) cells. Residual water as well as preextracted culture water containing P. piscicida cells induced c-fos-luciferase expressed in GH(4)C(1) cells with a rapid time course of induction and sensitive detection. The reporter gene assay detected activity in toxic isolates of P. piscicida from several North Carolina estuaries in 1997 and 1998 and may also be suitable for detecting toxic activity in human and animal serum.

Entities:  

Mesh：

Substances：

Year:  1999        PMID： 10464070      PMCID： PMC1566442          DOI： 10.1289/ehp.99107711

Source DB:  PubMed          Journal:  Environ Health Perspect        ISSN： 0091-6765            Impact factor:   9.031


  13 in total

1.  Domoic acid is a potent neurotoxin to neonatal rats.

Authors:  D Xi; Y G Peng; J S Ramsdell
Journal:  Nat Toxins       Date:  1997

2.  New 'phantom' dinoflagellate is the causative agent of major estuarine fish kills.

Authors:  J M Burkholder; E J Noga; C H Hobbs; H B Glasgow; S A Smith
Journal:  Nature       Date:  1992-07-30       Impact factor: 49.962

3.  Hypertension and identification of toxin in human urine and serum following a cluster of mussel-associated paralytic shellfish poisoning outbreaks.

Authors:  B D Gessner; P Bell; G J Doucette; E Moczydlowski; M A Poli; F Van Dolah; S Hall
Journal:  Toxicon       Date:  1997-05       Impact factor: 3.033

4.  Neuroexcitatory actions of ciguatoxin on brain regions associated with thermoregulation.

Authors:  Y G Peng; T B Taylor; R E Finch; P D Moeller; J S Ramsdell
Journal:  Neuroreport       Date:  1995-01-26       Impact factor: 1.837

5.  Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.

Authors:  T Mosmann
Journal:  J Immunol Methods       Date:  1983-12-16       Impact factor: 2.303

6.  Learning and memory difficulties after environmental exposure to waterways containing toxin-producing Pfiesteria or Pfiesteria-like dinoflagellates.

Authors:  L M Grattan; D Oldach; T M Perl; M H Lowitt; D L Matuszak; C Dickson; C Parrott; R C Shoemaker; C L Kauffman; M P Wasserman; J R Hebel; P Charache; J G Morris
Journal:  Lancet       Date:  1998-08-15       Impact factor: 79.321

7.  Brain Fos induction is a sensitive biomarker for the lowest observed neuroexcitatory effects of domoic acid.

Authors:  Y G Peng; J S Ramsdell
Journal:  Fundam Appl Toxicol       Date:  1996-06

8.  Tetrazolium-based cell bioassay for neurotoxins active on voltage-sensitive sodium channels: semiautomated assay for saxitoxins, brevetoxins, and ciguatoxins.

Authors:  R L Manger; L S Leja; S Y Lee; J M Hungerford; M M Wekell
Journal:  Anal Biochem       Date:  1993-10       Impact factor: 3.365

9.  Development of rapid and sensitive high throughput pharmacologic assays for marine phycotoxins.

Authors:  F M Van Dolah; E L Finley; B L Haynes; G J Doucette; P D Moeller; J S Ramsdell
Journal:  Nat Toxins       Date:  1994

10.  Neuroexcitatory and neurotoxic actions of the amnesic shellfish poison, domoic acid.

Authors:  Y G Peng; T B Taylor; R E Finch; R C Switzer; J S Ramsdell
Journal:  Neuroreport       Date:  1994-04-14       Impact factor: 1.837
View more
  17 in total

1.  Demonstration of toxicity to fish and to mammalian cells by Pfiesteria species: comparison of assay methods and strains.

Authors:  Joann M Burkholder; Andrew S Gordon; Peter D Moeller; J Mac Law; Kathryn J Coyne; Alan J Lewitus; John S Ramsdell; Harold G Marshall; Nora J Deamer; S Craig Cary; Jason W Kempton; Steven L Morton; Parke A Rublee
Journal:  Proc Natl Acad Sci U S A       Date:  2005-02-22       Impact factor: 11.205

2.  Development of real-time PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates.

Authors:  H A Bowers; T Tengs; H B Glasgow; J M Burkholder; P A Rublee; D W Oldach
Journal:  Appl Environ Microbiol       Date:  2000-11       Impact factor: 4.792

3.  Evaluation of cytotoxicity, morphological alterations and oxidative stress in Chinook salmon cells exposed to copper oxide nanoparticles.

Authors:  Koigoora Srikanth; Eduarda Pereira; Armando C Duarte; Janapala Venkateswara Rao
Journal:  Protoplasma       Date:  2015-06-27       Impact factor: 3.356

4.  Heteroduplex mobility assay-guided sequence discovery: elucidation of the small subunit (18S) rDNA sequences of Pfiesteria piscicida and related dinoflagellates from complex algal culture and environmental sample DNA pools.

Authors:  D W Oldach; C F Delwiche; K S Jakobsen; T Tengs; E G Brown; J W Kempton; E F Schaefer; H A Bowers; H B Glasgow; J M Burkholder; K A Steidinger; P A Rublee
Journal:  Proc Natl Acad Sci U S A       Date:  2000-04-11       Impact factor: 11.205

5.  Are Pfiesteria species toxicogenic? Evidence against production of ichthyotoxins by Pfiesteria shumwayae.

Authors:  J P Berry; K S Reece; K S Rein; D G Baden; L W Haas; W L Ribeiro; J D Shields; R V Snyder; W K Vogelbein; R E Gawley
Journal:  Proc Natl Acad Sci U S A       Date:  2002-08-05       Impact factor: 11.205

6.  Identification of a P2X7 receptor in GH(4)C(1) rat pituitary cells: a potential target for a bioactive substance produced by Pfiesteria piscicida.

Authors:  K L Kimm-Brinson; P D Moeller; M Barbier; H Glasgow; J M Burkholder; J S Ramsdell
Journal:  Environ Health Perspect       Date:  2001-05       Impact factor: 9.031

7.  Current progress in isolation and characterization of toxins isolated from Pfiesteria piscicida.

Authors:  P D Moeller; S L Morton; B A Mitchell; S K Sivertsen; E R Fairey; T M Mikulski; H Glasgow; N J Deamer-Melia; J M Burkholder; J S Ramsdell
Journal:  Environ Health Perspect       Date:  2001-10       Impact factor: 9.031

Review 8.  The standardized fish bioassay procedure for detecting and culturing actively toxic Pfiesteria, used by two reference laboratories for atlantic and gulf coast states.

Authors:  J M Burkholder; H G Marshall; H B Glasgow; D W Seaborn; N J Deamer-Melia
Journal:  Environ Health Perspect       Date:  2001-10       Impact factor: 9.031

9.  Environmental public health surveillance: possible estuary-associated syndrome.

Authors:  L C Backer; A S Niskar; C Rubin; K Blindauer; D Christianson; L Naeher; H S Rogers
Journal:  Environ Health Perspect       Date:  2001-10       Impact factor: 9.031

Review 10.  Pfiesteria: review of the science and identification of research gaps. Report for the National Center for Environmental Health, Centers for Disease Control and Prevention.

Authors:  J Samet; G S Bignami; R Feldman; W Hawkins; J Neff; T Smayda
Journal:  Environ Health Perspect       Date:  2001-10       Impact factor: 9.031
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.