Literature DB >> 10455237

Designer deletion and prototrophic strains derived from Saccharomyces cerevisiae strain W303-1a.

K Replogle1, L Hovland, D H Rivier.   

Abstract

We report the construction of Saccharomyces cerevisiae strains isogenic to W303-1a that are designed to allow efficient genetic analysis. To facilitate the generation of null alleles of target genes by PCR-mediated gene disruption, we constructed designer deletion alleles of the ARG4, TRP1 and URA3 genes. In addition, a single pair of oligonucleotide primers were designed that can be used to amplify any of several marker genes for use in PCR-mediated gene disruption. A new version of the 'reusable' hisG-URA3-hisG cassette was constructed for use in PCR-mediated gene disruption. Finally, to facilitate the formation of isogenic diploids by selection, we constructed strains that contain combinations of wild-type alleles of ADE2, HIS3, LEU2, TRP1 and URA3. Copyright 1999 John Wiley & Sons, Ltd.

Entities:  

Mesh：

Substances：

Year:  1999        PMID： 10455237     DOI： 10.1002/(SICI)1097-0061(199908)15:11<1141::AID-YEA439>3.0.CO;2-P

Source DB:  PubMed          Journal:  Yeast        ISSN： 0749-503X            Impact factor:   3.239


  8 in total

1.  Transferring whole genomes from bacteria to yeast spheroplasts using entire bacterial cells to reduce DNA shearing.

Authors:  Bogumil J Karas; Jelena Jablanovic; Edward Irvine; Lijie Sun; Li Ma; Philip D Weyman; Daniel G Gibson; John I Glass; J Craig Venter; Clyde A Hutchison; Hamilton O Smith; Yo Suzuki
Journal:  Nat Protoc       Date:  2014-03-06       Impact factor: 13.491

2.  A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast.

Authors:  U Gueldener; J Heinisch; G J Koehler; D Voss; J H Hegemann
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

3.  Rationally designed perturbation factor drives evolution in Saccharomyces cerevisiae for industrial application.

Authors:  Xin Xu; Chunfeng Liu; Chengtuo Niu; Jinjing Wang; Feiyun Zheng; Yongxian Li; Qi Li
Journal:  J Ind Microbiol Biotechnol       Date:  2018-08-03       Impact factor: 3.346

4.  The DNA end-binding protein Ku regulates silencing at the internal HML and HMR loci in Saccharomyces cerevisiae.

Authors:  Catherine L Vandre; Rohinton T Kamakaka; David H Rivier
Journal:  Genetics       Date:  2008-09-14       Impact factor: 4.562

5.  Multiple Antibiotic Resistance Plasmids Allow Scalable,
PCR-Mediated DNA Manipulation and Near-Zero Background Cloning.

Authors:  Remigiusz Arnak; Burcin Altun; Valentina Tosato; Carlo V Bruschi
Journal:  Food Technol Biotechnol       Date:  2016-09       Impact factor: 3.918

6.  Slx5 promotes transcriptional silencing and is required for robust growth in the absence of Sir2.

Authors:  Russell P Darst; Sandra N Garcia; Melissa R Koch; Lorraine Pillus
Journal:  Mol Cell Biol       Date:  2007-12-17       Impact factor: 4.272

7.  New and Redesigned pRS Plasmid Shuttle Vectors for Genetic Manipulation of Saccharomycescerevisiae.

Authors:  Mark K Chee; Steven B Haase
Journal:  G3 (Bethesda)       Date:  2012-05-01       Impact factor: 3.154

8.  A Saccharomyces eubayanus haploid resource for research studies.

Authors:  Jennifer Molinet; Kamila Urbina; Claudia Villegas; Valentina Abarca; Christian I Oporto; Pablo Villarreal; Carlos A Villarroel; Francisco Salinas; Roberto F Nespolo; Francisco A Cubillos
Journal:  Sci Rep       Date:  2022-04-08       Impact factor: 4.379
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.