Literature DB >> 10421050

Simple procedure for drug susceptibility testing of Mycobacterium tuberculosis using a commercial colorimetic assay.

J C Palomino1, F Portaels.   

Abstract

The aim of this study was to evaluate a simple method using a commercial colorimetric assay (Alamar Blue Oxidation-Reduction Indicator; Accumed, USA) in a microtiter format for testing the susceptibility of 94 strains of Mycobacterium tuberculosis to isoniazid, rifampicin, ethambutol and streptomycin. The method makes use of one critical concentration of each drug, and the results are available within 8-10 days. Overall, 97.1% agreement with the proportion method was obtained. Full agreement was obtained for isoniazid and rifampicin. The method is simple to perform, permits visual reading of results, and is practicable for laboratories with limited resources.

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Year:  1999        PMID: 10421050     DOI: 10.1007/pl00015025

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN: 0934-9723            Impact factor:   3.267


  14 in total

1.  Evaluation of mycobacteria growth indicator tube for direct and indirect drug susceptibility testing of Mycobacterium tuberculosis from respiratory specimens in a Siberian prison hospital.

Authors:  V Goloubeva; M Lecocq; P Lassowsky; F Matthys; F Portaels; I Bastian
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948

2.  Use of receiver operating characteristic curves to assess the performance of a microdilution assay for determination of drug susceptibility of clinical isolates of Mycobacterium tuberculosis.

Authors:  J Luna-Herrera; G Martínez-Cabrera; R Parra-Maldonado; J A Enciso-Moreno; J Torres-López; F Quesada-Pascual; R Delgadillo-Polanco; S G Franzblau
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2003-01-25       Impact factor: 3.267

3.  Kanamycin susceptibility testing of Mycobacterium tuberculosis using Mycobacterium Growth Indicator Tube and a colorimetric method.

Authors:  I Bastian; L Rigouts; J C Palomino; F Portaels
Journal:  Antimicrob Agents Chemother       Date:  2001-06       Impact factor: 5.191

4.  In vitro activities of cloxyquin (5-chloroquinolin-8-ol) against Mycobacterium tuberculosis.

Authors:  Poonpilas Hongmanee; Kamolchanok Rukseree; Benjamas Buabut; Boontiwa Somsri; Prasit Palittapongarnpim
Journal:  Antimicrob Agents Chemother       Date:  2006-12-18       Impact factor: 5.191

5.  Rapid and inexpensive drug susceptibility testing of Mycobacterium tuberculosis with a nitrate reductase assay.

Authors:  K A Kristian Angeby; Lisbeth Klintz; Sven E Hoffner
Journal:  J Clin Microbiol       Date:  2002-02       Impact factor: 5.948

6.  Validation of a rapid method for detection of M. tuberculosis resistance to isoniazid and rifampin in Lima, Peru.

Authors:  L A Solis; S S Shin; L L Han; F Llanos; M Stowell; A Sloutsky
Journal:  Int J Tuberc Lung Dis       Date:  2005-07       Impact factor: 2.373

7.  Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis.

Authors:  Juan-Carlos Palomino; Anandi Martin; Mirtha Camacho; Humberto Guerra; Jean Swings; Françoise Portaels
Journal:  Antimicrob Agents Chemother       Date:  2002-08       Impact factor: 5.191

8.  Broth microdilution susceptibility testing for Leptospira spp.

Authors:  Clinton K Murray; Duane R Hospenthal
Journal:  Antimicrob Agents Chemother       Date:  2004-05       Impact factor: 5.191

9.  Resazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple, and inexpensive method.

Authors:  Anandi Martin; Mirtha Camacho; Françoise Portaels; Juan Carlos Palomino
Journal:  Antimicrob Agents Chemother       Date:  2003-11       Impact factor: 5.191

10.  Rapid colorimetric method for testing susceptibility of Mycobacterium tuberculosis to isoniazid and rifampin in liquid cultures.

Authors:  H Syre; S Phyu; P Sandven; B Bjorvatn; H M S Grewal
Journal:  J Clin Microbiol       Date:  2003-11       Impact factor: 5.948

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