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Literature DB >> 11326024

Pretreatment with urea-hydrochloric acid enhances the isolation of Helicobacter pylori from contaminated specimens.

Q Song¹, G W Zirnstein, B Swaminathan, B D Gold.

Abstract

Human saliva seeded with H. pylori was incubated in urea-HCl and then cultured on nonselective media. Pretreatment with 0.06 N HCl-0.08 M urea for 5 min at 37 degrees C resulted in reproducible isolation of H. pylori, even at low inocula (< or =10(2) CFU/ml of saliva), despite the presence of large numbers of contaminating organisms.

Entities: Chemical Species

Mesh：

Substances：
Urea
Hydrochloric Acid

Year: 2001 PMID： 11326024 PMCID： PMC88059 DOI： 10.1128/JCM.39.5.1967-1968.2001

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

22 in total

1. Quantitation of Helicobacter pylori in dental plaque samples by competitive polymerase chain reaction.

Authors: Q Song; B Haller; D Ulrich; A Wichelhaus; G Adler; G Bode
Journal: J Clin Pathol Date: 2000-03 Impact factor: 3.411

2. A newly developed PCR assay of H. pylori in gastric biopsy, saliva, and feces. Evidence of high prevalence of H. pylori in saliva supports oral transmission.

Authors: C Li; T Ha; D A Ferguson; D S Chi; R Zhao; N R Patel; G Krishnaswamy; E Thomas
Journal: Dig Dis Sci Date: 1996-11 Impact factor: 3.199

3. Purification and N-terminal analysis of urease from Helicobacter pylori.

Authors: L T Hu; H L Mobley
Journal: Infect Immun Date: 1990-04 Impact factor: 3.441

4. NADH-Dependent coupled enzyme assay for urease and other ammonia-producing systems.

Authors: H Kaltwasser; H G Schlegel
Journal: Anal Biochem Date: 1966-07 Impact factor: 3.365

5. Studies on the intestinal flora. I. The bacterial flora of the gastrointestinal tract in healthy and achlorhydric persons.

Authors: B S Drasar; M Shiner; G M McLeod
Journal: Gastroenterology Date: 1969-01 Impact factor: 22.682

Pretreatment with urea-hydrochloric acid enhances the isolation of Helicobacter pylori from contaminated specimens.

1. Quantitation of Helicobacter pylori in dental plaque samples by competitive polymerase chain reaction.

2. A newly developed PCR assay of H. pylori in gastric biopsy, saliva, and feces. Evidence of high prevalence of H. pylori in saliva supports oral transmission.

3. Purification and N-terminal analysis of urease from Helicobacter pylori.

4. NADH-Dependent coupled enzyme assay for urease and other ammonia-producing systems.

5. Studies on the intestinal flora. I. The bacterial flora of the gastrointestinal tract in healthy and achlorhydric persons.

6. Absence of Helicobacter pylori in dental plaques in Helicobacter pylori positive dyspeptic patients.

7. Effect of omeprazole on intragastric bacterial counts, nitrates, nitrites, and N-nitroso compounds.

8. Isolation of Helicobacter pylori from saliva.

9. Helicobacter pylori requires an acidic environment to survive in the presence of urea.

10. Influence of oropharyngeal flora and specimen pretreatment on the recovery of Helicobacter pylori.

Review 1. Are the view of Helicobacter pylori colonized in the oral cavity an illusion?