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Literature DB >> 10359801

An experimental solution for the Luria-Delbrück fluctuation problem in measuring hypermutation rates.

J Bachl¹, M Dessing, C Olsson, R C von Borstel, C Steinberg.

Abstract

A cell line harboring all trans-acting elements necessary for hypermutation was transfected with a plasmid harboring the major cis-acting elements plus a green fluorescent protein gene containing a premature chain-termination codon. Transfected cells do not fluoresce unless the stop codon reverts. When a sizable cell population is purged of revertants by sorting, the frequency of mutants increases linearly with time, and there is no Luria-Delbrück fluctuation effect. Moreover, as mutant frequencies seemed to vary less than cell numbers in replicate cultures, it is suggested that hypermutation might not be coupled closely to cell division.

Entities: Disease

Mesh：

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Year: 1999 PMID： 10359801 PMCID： PMC22004 DOI： 10.1073/pnas.96.12.6847

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

19 in total

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9 in total

Review 1. Sorting out mutation rates.

Authors: P L Foster
Journal: Proc Natl Acad Sci U S A Date: 1999-07-06 Impact factor: 11.205

An experimental solution for the Luria-Delbrück fluctuation problem in measuring hypermutation rates.

1. The Direct Estimation of Mutation Rate from Mutant Frequency under Special Conditions.

2. Direct demonstration of the mutagenic action of euflavine on baker's yeast.

3. Description of the chemostat.

4. Enhancers of hypermutation.

Review 5. Mismatch repair as a source of mutations in non-dividing cells.

6. Transgenic Chinese hamster V79 cell lines which exhibit variable levels of gpt mutagenesis.

7. Measuring spontaneous mutation rates in yeast.

8. Green fluorescent protein as a marker for gene expression.

9. Modeling and measurement of the spontaneous mutation rate in mammalian cells.

10. Aequorea green fluorescent protein analysis by flow cytometry.

Review 1. Sorting out mutation rates.

2. Memories of a mentor: Charley Steinberg.

3. Methods for determining spontaneous mutation rates.

4. Proliferation model dependence in fluctuation analysis: the neutral case.

5. AID-GFP chimeric protein increases hypermutation of Ig genes with no evidence of nuclear localization.

6. Reduced rates of gene loss, gene silencing, and gene mutation in Dnmt1-deficient embryonic stem cells.

7. A quantitative analysis of genomic instability in lymphoid and plasma cell neoplasms based on the PIG-A gene.

8. A Framework for Investigating Rules of Life by Establishing Zones of Influence.

9. The rate of mutation of a single gene.