Literature DB >> 10329779

Taking MAD to the extreme: ultrafast protein structure determination.

M A Walsh1, I Dementieva, G Evans, R Sanishvili, A Joachimiak.   

Abstract

Multiwavelength anomalous diffraction data were measured in 23 min from a 16 kDa selenomethionyl substituted protein, producing experimental phases to 2.25 A resolution. The data were collected on a mosaic 3 x 3 charge-coupled device using undulator radiation from the Structural Biology Center 19ID beamline at the Argonne National Laboratory's Advanced Photon Source. The phases were independently obtained semiautomatically by two crystallographic program suites, CCP4 and CNS. The quality and speed of this data acquisition exemplify the opportunities at third-generation synchrotron sources for high-throughput protein crystal structure determination.

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Year:  1999        PMID： 10329779     DOI： 10.1107/s0907444999003698

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  62 in total

1.  Structure of cyanase reveals that a novel dimeric and decameric arrangement of subunits is required for formation of the enzyme active site.

Authors:  M A Walsh; Z Otwinowski; A Perrakis; P M Anderson; A Joachimiak
Journal:  Structure       Date:  2000-05-15       Impact factor: 5.006

2.  Structure of RsrI methyltransferase, a member of the N6-adenine beta class of DNA methyltransferases.

Authors:  R D Scavetta; C B Thomas; M A Walsh; S Szegedi; A Joachimiak; R I Gumport; M E Churchill
Journal:  Nucleic Acids Res       Date:  2000-10-15       Impact factor: 16.971

3.  Streptococcus pneumonia YlxR at 1.35 A shows a putative new fold.

Authors:  J Osipiuk; P Górnicki; L Maj; I Dementieva; R Laskowski; A Joachimiak
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2001-10-25

4.  Autotracing of Escherichia coli acetate CoA-transferase alpha-subunit structure using 3.4 A MAD and 1.9 A native data.

Authors:  S Korolev; O Koroleva; K Petterson; M Gu; F Collart; I Dementieva; A Joachimiak
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2002-11-23

5.  Deep trefoil knot implicated in RNA binding found in an archaebacterial protein.

Authors:  Thomas I Zarembinski; Youngchang Kim; Kelly Peterson; Dinesh Christendat; Akil Dharamsi; Cheryl H Arrowsmith; Aled M Edwards; Andrzej Joachimiak
Journal:  Proteins       Date:  2003-02-01

6.  Crystal structure of Escherichia coli EC1530, a glyoxylate induced protein YgbM.

Authors:  Y Kim; T Skarina; S Beasley; R Laskowski; C Arrowsmith; A Joachimiak; A Edwards; A Savchenko
Journal:  Proteins       Date:  2002-08-01

7.  Crystal structure of Bacillus subtilis ioli shows endonuclase IV fold with altered Zn binding.

Authors:  R-G Zhang; I Dementieva; N Duke; F Collart; E Quaite-Randall; R Alkire; L Dieckman; N Maltsev; O Korolev; A Joachimiak
Journal:  Proteins       Date:  2002-08-01

8.  Structure of Escherichia coli ribose-5-phosphate isomerase: a ubiquitous enzyme of the pentose phosphate pathway and the Calvin cycle.

Authors:  Rong guang Zhang; C Evalena Andersson; Alexei Savchenko; Tatiana Skarina; Elena Evdokimova; Steven Beasley; Cheryl H Arrowsmith; Aled M Edwards; Andrzej Joachimiak; Sherry L Mowbray
Journal:  Structure       Date:  2003-01       Impact factor: 5.006

9.  Crystal structure of YfeU protein from Haemophilus influenzae: a predicted etherase involved in peptidoglycan recycling.

Authors:  Y Kim; P Quartey; R Ng; T I Zarembinski; A Joachimiak
Journal:  J Struct Funct Genomics       Date:  2009-02-21

10.  Structural and functional analysis of AsbF: origin of the stealth 3,4-dihydroxybenzoic acid subunit for petrobactin biosynthesis.

Authors:  Brian F Pfleger; Youngchang Kim; Tyler D Nusca; Natalia Maltseva; Jung Yeop Lee; Christopher M Rath; Jamie B Scaglione; Brian K Janes; Erica C Anderson; Nicholas H Bergman; Philip C Hanna; Andrzej Joachimiak; David H Sherman
Journal:  Proc Natl Acad Sci U S A       Date:  2008-10-27       Impact factor: 11.205
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