Literature DB >> 9658154

Production of uninfectious human immunodeficiency virus type 1 containing viral protein R fused to a single-chain antibody against viral integrase.

N Okui1, N Kobayashi, Y Kitamura.   

Abstract

A single-chain antibody (scAb) against human immunodeficiency virus type 1 (HIV-1) integrase was expressed as a fusion protein of scAb and HIV-1 viral protein R (Vpr), together with the HIV-1 genome, in human 293T cells. The expression did not affect virion production much but markedly reduced the infectivity of progeny virions. The fusion protein was found to be incorporated into the virions. The incorporation appears to account for the reduced infectivity.

Entities:  

Mesh：

Substances：

Year:  1998        PMID： 9658154      PMCID： PMC109914     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  32 in total

1.  Efficient selection for high-expression transfectants with a novel eukaryotic vector.

Authors:  H Niwa; K Yamamura; J Miyazaki
Journal:  Gene       Date:  1991-12-15       Impact factor: 3.688

2.  Changes in growth properties on passage in tissue culture of viruses derived from infectious molecular clones of HIV-1LAI, HIV-1MAL, and HIV-1ELI.

Authors:  K Peden; M Emerman; L Montagnier
Journal:  Virology       Date:  1991-12       Impact factor: 3.616

3.  Identification of residues in the N-terminal acidic domain of HIV-1 Vpr essential for virion incorporation.

Authors:  S Mahalingam; S A Khan; M A Jabbar; C E Monken; R G Collman; A Srinivasan
Journal:  Virology       Date:  1995-02-20       Impact factor: 3.616

4.  Association of integrase, matrix, and reverse transcriptase antigens of human immunodeficiency virus type 1 with viral nucleic acids following acute infection.

Authors:  M I Bukrinsky; N Sharova; T L McDonald; T Pushkarskaya; W G Tarpley; M Stevenson
Journal:  Proc Natl Acad Sci U S A       Date:  1993-07-01       Impact factor: 11.205

5.  The Vpr protein of human immunodeficiency virus type 1 influences nuclear localization of viral nucleic acids in nondividing host cells.

Authors:  N K Heinzinger; M I Bukrinsky; S A Haggerty; A M Ragland; V Kewalramani; M A Lee; H E Gendelman; L Ratner; M Stevenson; M Emerman
Journal:  Proc Natl Acad Sci U S A       Date:  1994-07-19       Impact factor: 11.205

6.  Human immunodeficiency virus type 1 viral protein R localization in infected cells and virions.

Authors:  Y L Lu; P Spearman; L Ratner
Journal:  J Virol       Date:  1993-11       Impact factor: 5.103

7.  Intracellular expression of antibody fragments directed against HIV reverse transcriptase prevents HIV infection in vitro.

Authors:  J P Maciejewski; F F Weichold; N S Young; A Cara; D Zella; M S Reitz; R C Gallo
Journal:  Nat Med       Date:  1995-07       Impact factor: 53.440

8.  Targeting a foreign protein into virion particles by fusion with the Vpx protein of simian immunodeficiency virus.

Authors:  I W Park; J Sodroski
Journal:  J Acquir Immune Defic Syndr Hum Retrovirol       Date:  1996-04-01

9.  Protein stability influences human immunodeficiency virus type 2 Vpr virion incorporation and cell cycle effect.

Authors:  V N Kewalramani; C S Park; P A Gallombardo; M Emerman
Journal:  Virology       Date:  1996-04-15       Impact factor: 3.616

10.  Targeting foreign proteins to human immunodeficiency virus particles via fusion with Vpr and Vpx.

Authors:  X Wu; H Liu; H Xiao; J Kim; P Seshaiah; G Natsoulis; J D Boeke; B H Hahn; J C Kappes
Journal:  J Virol       Date:  1995-06       Impact factor: 5.103
View more
  4 in total

1.  Monoclonal antibodies against the minimal DNA-binding domain in the carboxyl-terminal region of human immunodeficiency virus type 1 integrase.

Authors:  T Ishikawa; N Okui; N Kobayashi; R Sakuma; T Kitamura; Y Kitamura
Journal:  J Virol       Date:  1999-05       Impact factor: 5.103

2.  Functional role of residues corresponding to helical domain II (amino acids 35 to 46) of human immunodeficiency virus type 1 Vpr.

Authors:  S P Singh; B Tomkowicz; D Lai; M Cartas; S Mahalingam; V S Kalyanaraman; R Murali; A Srinivasan
Journal:  J Virol       Date:  2000-11       Impact factor: 5.103

3.  Engineering and functional evaluation of a single-chain antibody against HIV-1 external glycoprotein gp120.

Authors:  H W Wang; D Cole; W Z Jiang; H T Jin; N Fu; Z L Chen; N Y Jin
Journal:  Clin Exp Immunol       Date:  2005-07       Impact factor: 4.330

Review 4.  Capsid-Targeted Viral Inactivation: A Novel Tactic for Inhibiting Replication in Viral Infections.

Authors:  Xingcui Zhang; Renyong Jia; Jiakun Zhou; Mingshu Wang; Zhongqiong Yin; Anchun Cheng
Journal:  Viruses       Date:  2016-09-21       Impact factor: 5.048
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.