Literature DB >> 10094711

Target joining of duplicated insertion sequence IS21 is assisted by IstB protein in vitro.

S Schmid1, B Berger, D Haas.   

Abstract

Tandemly repeated insertion sequence IS21, located on a suicide plasmid, promoted replicon fusion with bacteriophage lambda in vitro in the presence of ATP. This reaction was catalyzed in a cell extract containing the 45-kDa IstA protein (cointegrase) and the 30-kDa IstB helper protein of IS21 after both proteins had been overproduced in Escherichia coli. Without IstB, replicon fusion was inefficient and did not produce the 4-bp target duplications typical of IS21.

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Year:  1999        PMID: 10094711      PMCID: PMC93646     

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  24 in total

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Authors:  P O Brown; B Bowerman; H E Varmus; J M Bishop
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Authors:  R M Chalmers; N Kleckner
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4.  Mode of replicon fusion mediated by the duplicated insertion sequence IS21 in Escherichia coli.

Authors:  C Reimmann; D Haas
Journal:  Genetics       Date:  1987-04       Impact factor: 4.562

5.  In vitro transposition of transposon Tn3.

Authors:  H Ichikawa; E Ohtsubo
Journal:  J Biol Chem       Date:  1990-11-05       Impact factor: 5.157

6.  The DNA intermediate in yeast Ty1 element transposition copurifies with virus-like particles: cell-free Ty1 transposition.

Authors:  D J Eichinger; J D Boeke
Journal:  Cell       Date:  1988-09-23       Impact factor: 41.582

7.  Genetic structure, function and regulation of the transposable element IS21.

Authors:  C Reimmann; R Moore; S Little; A Savioz; N S Willetts; D Haas
Journal:  Mol Gen Genet       Date:  1989-02

8.  Tn5supF, a 264-base-pair transposon derived from Tn5 for insertion mutagenesis and sequencing DNAs cloned in phage lambda.

Authors:  S H Phadnis; H V Huang; D E Berg
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205

9.  The istA gene of insertion sequence IS21 is essential for cleavage at the inner 3' ends of tandemly repeated IS21 elements in vitro.

Authors:  C Reimmann; D Haas
Journal:  EMBO J       Date:  1990-12       Impact factor: 11.598

10.  The phage Mu transpososome core: DNA requirements for assembly and function.

Authors:  H Savilahti; P A Rice; K Mizuuchi
Journal:  EMBO J       Date:  1995-10-02       Impact factor: 11.598

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5.  Unstable Mechanisms of Resistance to Inhibitors of Escherichia coli Lipoprotein Signal Peptidase.

Authors:  Homer Pantua; Elizabeth Skippington; Marie-Gabrielle Braun; Cameron L Noland; Jingyu Diao; Yutian Peng; Susan L Gloor; Donghong Yan; Jing Kang; Anand Kumar Katakam; Janina Reeder; Georgette M Castanedo; Keira Garland; Laszlo Komuves; Meredith Sagolla; Cary D Austin; Jeremy Murray; Yiming Xu; Zora Modrusan; Min Xu; Emily J Hanan; Sharookh B Kapadia
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