| Literature DB >> 10094711 |
Abstract
Tandemly repeated insertion sequence IS21, located on a suicide plasmid, promoted replicon fusion with bacteriophage lambda in vitro in the presence of ATP. This reaction was catalyzed in a cell extract containing the 45-kDa IstA protein (cointegrase) and the 30-kDa IstB helper protein of IS21 after both proteins had been overproduced in Escherichia coli. Without IstB, replicon fusion was inefficient and did not produce the 4-bp target duplications typical of IS21.Entities:
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Year: 1999 PMID: 10094711 PMCID: PMC93646
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490