Literature DB >> 10078866

Structure of a Lys49-phospholipase A2 homologue isolated from the venom of Bothrops nummifer (jumping viper).

W F de Azevedo1, R J Ward, J M Gutiérrez, R K Arni.   

Abstract

Lys49-Phospholipase A2 (Lys49-PLA2) homologues damage membranes by a Ca2+-independent mechanism which does not involve catalytic activity. We have solved the structure of myotoxin-I, a Lys49-PLA2 homologue isolated from the venom of Bothrops nummifer (jumping viper) at 2.4 A resolution using molecular replacement techniques. The final model has been refined to a final R-factor of 18.4% (R-free = 23.2%), and shows excellent geometry. The myotoxin-I from Bothrops nummifer is dimeric in the crystalline state as has been observed for other Lys49-PLA2 homologues. In addition, a continuous electron density in the active site and substrate binding channel could be successfully modeled as a fatty-acid molecule.

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Year:  1999        PMID： 10078866     DOI： 10.1016/s0041-0101(98)00189-5

Source DB:  PubMed          Journal:  Toxicon        ISSN： 0041-0101            Impact factor:   3.033


  7 in total

1.  Crystallization and preliminary X-ray diffraction analysis of a myotoxic Lys49-PLA2 from Bothrops jararacussu venom complexed with p-bromophenacyl bromide.

Authors:  D P Marchi-Salvador; C A H Fernandes; S F Amui; A M Soares; M R M Fontes
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-05-31

2.  Crystal structure of the complex formed between a group I phospholipase A2 and a naturally occurring fatty acid at 2.7 A resolution.

Authors:  Garima Singh; Jayasankar Jasti; K Saravanan; Sujata Sharma; Punit Kaur; A Srinivasan; Tej P Singh
Journal:  Protein Sci       Date:  2005-02       Impact factor: 6.725

3.  Crystallization and preliminary X-ray diffraction analysis of myotoxin I, a Lys49-phospholipase A2 from Bothrops moojeni.

Authors:  D P Marchi-Salvador; L B Silveira; A M Soares; M R M Fontes
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-09-13

4.  Structural characterization of myotoxic ecarpholin S from Echis carinatus venom.

Authors:  Xingding Zhou; Tien-Chye Tan; S Valiyaveettil; Mei Lin Go; R Manjunatha Kini; Adrian Velazquez-Campoy; J Sivaraman
Journal:  Biophys J       Date:  2008-06-27       Impact factor: 4.033

5.  Structure of myotoxin II, a catalytically inactive Lys49 phospholipase A2 homologue from Atropoides nummifer venom.

Authors:  Mário T Murakami; Cristiane C Melo; Yamileth Angulo; Bruno Lomonte; Raghuvir K Arni
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-04-12

6.  Active-site mutagenesis of a Lys49-phospholipase A2: biological and membrane-disrupting activities in the absence of catalysis.

Authors:  Richard J Ward; Lucimara Chioato; Arthur H C de Oliveira; Roberto Ruller; Juliana M Sá
Journal:  Biochem J       Date:  2002-02-15       Impact factor: 3.857

7.  Crystallization and preliminary X-ray diffraction studies of BmooPLA2-I, a platelet-aggregation inhibitor and hypotensive phospholipase A2 from Bothrops moojeni venom.

Authors:  Guilherme H M Salvador; Daniela P Marchi-Salvador; Lucas B Silveira; Andreimar M Soares; Marcos R M Fontes
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-07-19
  7 in total

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