Literature DB >> 16511185

Crystallization and preliminary X-ray diffraction analysis of myotoxin I, a Lys49-phospholipase A2 from Bothrops moojeni.

D P Marchi-Salvador1, L B Silveira, A M Soares, M R M Fontes.   

Abstract

A new myotoxic Lys49-phospholipase A2 isolated from Bothrops moojeni snake venom has been crystallized. The crystals diffracted to 2.18 A resolution using a synchrotron-radiation source and belong to space group C2. The unit-cell parameters are a = 56.8, b = 125.0, c = 64.7 A, beta = 105.5 degrees. Preliminary analysis indicates the presence of four molecules in the asymmetric unit. This may suggest a new quaternary structure for this Lys49-phospholipase A2 in contrast to the dimeric and monomeric structures solved so far for this class of proteins.

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Year:  2005        PMID： 16511185      PMCID： PMC1991306          DOI： 10.1107/S174430910502717X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  21 in total

1.  Structural and functional characterization of myotoxin I, a Lys49 phospholipase A(2) homologue from Bothrops moojeni (Caissaca) snake venom.

Authors:  A M Soares; S H Andrião-Escarso; Y Angulo; B Lomonte; J M Gutiérrez; S Marangoni; M H Toyama; R K Arni; J R Giglio
Journal:  Arch Biochem Biophys       Date:  2000-01-01       Impact factor: 4.013

2.  Structure of a Lys49-phospholipase A2 homologue isolated from the venom of Bothrops nummifer (jumping viper).

Authors:  W F de Azevedo; R J Ward; J M Gutiérrez; R K Arni
Journal:  Toxicon       Date:  1999-02       Impact factor: 3.033

3.  A clinical and epidemiologic study of 292 cases of lance-headed viper bite in a Brazilian teaching hospital.

Authors:  S de A Nishioka; P V Silveira
Journal:  Am J Trop Med Hyg       Date:  1992-12       Impact factor: 2.345

4.  Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani.

Authors:  R K Arni; M R Fontes; C Barberato; J M Gutiérrez; C Díaz; R J Ward
Journal:  Arch Biochem Biophys       Date:  1999-06-15       Impact factor: 4.013

Review 5.  Lysine 49 phospholipase A2 proteins.

Authors:  C L Ownby; H S Selistre de Araujo; S P White; J E Fletcher
Journal:  Toxicon       Date:  1999-03       Impact factor: 3.033

6.  Structural basis for low catalytic activity in Lys49 phospholipases A2--a hypothesis: the crystal structure of piratoxin II complexed to fatty acid.

Authors:  W H Lee; M T da Silva Giotto; S Marangoni; M H Toyama; I Polikarpov; R C Garratt
Journal:  Biochemistry       Date:  2001-01-09       Impact factor: 3.162

Review 7.  What can venom phospholipases A(2) tell us about the functional diversity of mammalian secreted phospholipases A(2)?

Authors:  E Valentin; G Lambeau
Journal:  Biochimie       Date:  2000 Sep-Oct       Impact factor: 4.079

Review 8.  The expanding superfamily of phospholipase A(2) enzymes: classification and characterization.

Authors:  D A Six; E A Dennis
Journal:  Biochim Biophys Acta       Date:  2000-10-31

9.  Calcium ion independent membrane leakage induced by phospholipase-like myotoxins.

Authors:  S Rufini; P Cesaroni; A Desideri; R Farias; F Gubensek; J M Gutiérrez; P Luly; R Massoud; R Morero; J Z Pedersen
Journal:  Biochemistry       Date:  1992-12-15       Impact factor: 3.162

10.  Crystal structures of BnSP-7 and BnSP-6, two Lys49-phospholipases A(2): quaternary structure and inhibition mechanism insights.

Authors:  Angelo J Magro; Andreimar M Soares; José R Giglio; Marcos R M Fontes
Journal:  Biochem Biophys Res Commun       Date:  2003-11-21       Impact factor: 3.575
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  4 in total

1.  Crystallization and preliminary X-ray diffraction analysis of a myotoxic Lys49-PLA2 from Bothrops jararacussu venom complexed with p-bromophenacyl bromide.

Authors:  D P Marchi-Salvador; C A H Fernandes; S F Amui; A M Soares; M R M Fontes
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-05-31

2.  Preliminary X-ray crystallographic studies of BthTX-II, a myotoxic Asp49-phospholipase A2 with low catalytic activity from Bothrops jararacussu venom.

Authors:  L C Corrêa; D P Marchi-Salvador; A C O Cintra; A M Soares; M R M Fontes
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-07-24

3.  Therapeutic application of natural inhibitors against snake venom phospholipase A(2).

Authors:  Ramar Perumal Samy; Ponnampalam Gopalakrishnakone; Vincent Tk Chow
Journal:  Bioinformation       Date:  2012-01-06

4.  Structural and phylogenetic studies with MjTX-I reveal a multi-oligomeric toxin--a novel feature in Lys49-PLA2s protein class.

Authors:  Guilherme H M Salvador; Carlos A H Fernandes; Angelo J Magro; Daniela P Marchi-Salvador; Walter L G Cavalcante; Roberto M Fernandez; Márcia Gallacci; Andreimar M Soares; Cristiano L P Oliveira; Marcos R M Fontes
Journal:  PLoS One       Date:  2013-04-03       Impact factor: 3.240
  4 in total

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