Literature DB >> 11053399

Second-site suppressor mutations of inactivating substitutions at gly247 of the tetracycline efflux protein, Tet(B).

C A Saraceni-Richards1, S B Levy.   

Abstract

An Asp or Asn substitution for Gly247 in transmembrane helix 8 (TM-8) of Tet(B), the tetracycline efflux protein, eliminated tetracycline resistance. Second site suppressor mutations which partially restored resistance were located in TM-5, -8, -10, or -11 or in cytoplasmic loop 8-9 or loop 10-11. These results indicate physical proximity or functional relationships between TM-8 and these other regions of Tet(B).

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Year:  2000        PMID： 11053399      PMCID： PMC94801          DOI： 10.1128/JB.182.22.6514-6516.2000

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  23 in total

1.  Transmembrane remote conformational suppression of the Gly-332 mutation of the Tn10-encoded metal-tetracycline/H+ antiporter.

Authors:  T Kawabe; A Yamaguchi
Journal:  FEBS Lett       Date:  1999-08-20       Impact factor: 4.124

2.  The TetA(K) tetracycline/H(+) antiporter from Staphylococcus aureus: mutagenesis and functional analysis of motif C.

Authors:  S L Ginn; M H Brown; R A Skurray
Journal:  J Bacteriol       Date:  2000-03       Impact factor: 3.490

3.  Tertiary contacts of helix V in the lactose permease determined by site-directed chemical cross-linking in situ.

Authors:  J Wu; D Hardy; H R Kaback
Journal:  Biochemistry       Date:  1999-02-23       Impact factor: 3.162

4.  Nomenclature for new tetracycline resistance determinants.

Authors:  S B Levy; L M McMurry; T M Barbosa; V Burdett; P Courvalin; W Hillen; M C Roberts; J I Rood; D E Taylor
Journal:  Antimicrob Agents Chemother       Date:  1999-06       Impact factor: 5.191

5.  An analysis of suppressor mutations suggests that the two halves of the lactose permease function in a symmetrical manner.

Authors:  N J Pazdernik; S M Cain; R J Brooker
Journal:  J Biol Chem       Date:  1997-10-17       Impact factor: 5.157

6.  Cysteine-scanning mutagenesis around transmembrane segments 1 and 11 and their flanking loop regions of Tn10-encoded metal-Tetracycline/H+ antiporter.

Authors:  T Kimura-Someya; S Iwaki; S Konishi; N Tamura; Y Kubo; A Yamaguchi
Journal:  J Biol Chem       Date:  2000-06-23       Impact factor: 5.157

7.  Interaction between residues Glu269 (helix VIII) and His322 (helix X) of the lactose permease of Escherichia coli is essential for substrate binding.

Authors:  M M He; H R Kaback
Journal:  Biochemistry       Date:  1997-11-04       Impact factor: 3.162

8.  Evidence for interactions between helices 5 and 8 and a role for the interdomain loop in tetracycline resistance mediated by hybrid Tet proteins.

Authors:  C A Saraceni-Richards; S B Levy
Journal:  J Biol Chem       Date:  2000-03-03       Impact factor: 5.157

9.  Functional importance and local environments of the cysteines in the tetracycline resistance protein encoded by plasmid pBR322.

Authors:  J E Jewell; J Orwick; J Liu; K W Miller
Journal:  J Bacteriol       Date:  1999-03       Impact factor: 3.490

10.  Characterization of Glu126 and Arg144, two residues that are indispensable for substrate binding in the lactose permease of Escherichia coli.

Authors:  M Sahin-Tóth; J le Coutre; D Kharabi; G le Maire; J C Lee; H R Kaback
Journal:  Biochemistry       Date:  1999-01-12       Impact factor: 3.162
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  3 in total

Review 1.  Biochemistry of bacterial multidrug efflux pumps.

Authors:  Sanath Kumar; Manuel F Varela
Journal:  Int J Mol Sci       Date:  2012-04-10       Impact factor: 6.208

Review 2.  Multidrug efflux pumps from Enterobacteriaceae, Vibrio cholerae and Staphylococcus aureus bacterial food pathogens.

Authors:  Jody L Andersen; Gui-Xin He; Prathusha Kakarla; Ranjana K C; Sanath Kumar; Wazir Singh Lakra; Mun Mun Mukherjee; Indrika Ranaweera; Ugina Shrestha; Thuy Tran; Manuel F Varela
Journal:  Int J Environ Res Public Health       Date:  2015-01-28       Impact factor: 3.390

Review 3.  Functional and Structural Roles of the Major Facilitator Superfamily Bacterial Multidrug Efflux Pumps.

Authors:  Sanath Kumar; Manjusha Lekshmi; Ammini Parvathi; Manisha Ojha; Nicholas Wenzel; Manuel F Varela
Journal:  Microorganisms       Date:  2020-02-16
  3 in total

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