The antiviral activity of isatin beta-thiosemicarbazone derivatives on vaccinia virus infection in mice.

Newly synthetized compounds of Mannich bases isatin-beta-thiosemicarbazone derivatives: N,N'-bis-(beta-thiosemicarbazone-isatinmethyl)-2-methylpiperazine (TSKI-VI); N,N'-bis (beta-thiosemicarbazoneisatinmethyl)-cis-2,5-dimethylpiperazine (TSKI-VII) and N,N'-bis (beta-thiosemicarbazoneisatinmethyl)-trans-2,5-dimethylpiperazine (TSKI-VII), showed protective effect on mice infected i.c. with neurovaccinia virus. Antiviral activity was shown already at doses of 1-25 mg/kg increased with the concentrations of the administratered compounds. TSKI-VI given prophylactically also showed protective effect, similar to metisazone in mice later infected i.c. with vaccinia virus.