Literature DB >> 9929001

Purification of histidine tagged bacteriorhodopsin, pharaonis halorhodopsin and pharaonis sensory rhodopsin II functionally expressed in Escherichia coli.

I P Hohenfeld1, A A Wegener, M Engelhard.   

Abstract

Bacteriorhodopsin (BR) from Halobacterium salinarum as well as halorhodopsin (pHR) and sensory rhodopsin II (pSRII) from Natronobacterium pharaonis were functionally expressed in E. coli using the method of Shimono et al. IFEBS Lett. (1997) 420, 54-56]. The histidine tagged proteins were purified with yields up to 1.0 mg/l cell culture and characterized by ESI mass spectrometry and their photocycle. The pSRII and pHR photocycles were indistinguishable from the wild type proteins. The BR photocycle was considerably prolonged. pSOII is located in the cytoplasmic membrane and the C-terminus is oriented towards the cytoplasm as determined by immunogold labelling.

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Year:  1999        PMID： 9929001     DOI： 10.1016/s0014-5793(98)01659-7

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  36 in total

1.  X-ray structure of sensory rhodopsin II at 2.1-A resolution.

Authors:  A Royant; P Nollert; K Edman; R Neutze; E M Landau; E Pebay-Peyroula; J Navarro
Journal:  Proc Natl Acad Sci U S A       Date:  2001-08-14       Impact factor: 11.205

2.  Probing the proton channel and the retinal binding site of Natronobacterium pharaonis sensory rhodopsin II.

Authors:  Johann P Klare; Georg Schmies; Igor Chizhov; Kazumi Shimono; Naoki Kamo; Martin Engelhard
Journal:  Biophys J       Date:  2002-04       Impact factor: 4.033

3.  Static and time-resolved step-scan Fourier transform infrared investigations of the photoreaction of halorhodopsin from Natronobacterium pharaonis: consequences for models of the anion translocation mechanism.

Authors:  C Hackmann; J Guijarro; I Chizhov; M Engelhard; C Rödig; F Siebert
Journal:  Biophys J       Date:  2001-07       Impact factor: 4.033

4.  Structural insights into the early steps of receptor-transducer signal transfer in archaeal phototaxis.

Authors:  A A Wegener; J P Klare; M Engelhard; H J Steinhoff
Journal:  EMBO J       Date:  2001-10-01       Impact factor: 11.598

5.  Sensory rhodopsin II from the haloalkaliphilic natronobacterium pharaonis: light-activated proton transfer reactions.

Authors:  G Schmies; B Lüttenberg; I Chizhov; M Engelhard; A Becker; E Bamberg
Journal:  Biophys J       Date:  2000-02       Impact factor: 4.033

6.  Electric-field dependent decays of two spectroscopically different M-states of photosensory rhodopsin II from Natronobacterium pharaonis.

Authors:  Laura Rivas; Silke Hippler-Mreyen; Martin Engelhard; Peter Hildebrandt
Journal:  Biophys J       Date:  2003-06       Impact factor: 4.033

7.  The photochemical reaction cycle and photoinduced proton transfer of sensory rhodopsin II (Phoborhodopsin) from Halobacterium salinarum.

Authors:  Jun Tamogami; Takashi Kikukawa; Yoichi Ikeda; Ayaka Takemura; Makoto Demura; Naoki Kamo
Journal:  Biophys J       Date:  2010-04-07       Impact factor: 4.033

8.  Rapid separation of bacteriorhodopsin using a laminar-flow extraction system in a microfluidic device.

Authors:  Yun Suk Huh; Chang-Moon Jeong; Ho Nam Chang; Sang Yup Lee; Won Hi Hong; Tae Jung Park
Journal:  Biomicrofluidics       Date:  2010-01-27       Impact factor: 2.800

9.  Improving yields of deuterated, methyl labeled protein by growing in H2O.

Authors:  Evan S O'Brien; Danny W Lin; Brian Fuglestad; Matthew A Stetz; Travis Gosse; Cecilia Tommos; A Joshua Wand
Journal:  J Biomol NMR       Date:  2018-08-02       Impact factor: 2.835

10.  Role of Asp193 in chromophore-protein interaction of pharaonis phoborhodopsin (sensory rhodopsin II).

Authors:  Masayuki Iwamoto; Yuji Furutani; Yuki Sudo; Kazumi Shimono; Hideki Kandori; Naoki Kamo
Journal:  Biophys J       Date:  2002-08       Impact factor: 4.033
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