Literature DB >> 9925634

Detection of shiga-like toxin (stx1 and stx2), intimin (eaeA), and enterohemorrhagic Escherichia coli (EHEC) hemolysin (EHEC hlyA) genes in animal feces by multiplex PCR.

P K Fagan1, M A Hornitzky, K A Bettelheim, S P Djordjevic.   

Abstract

A multiplex PCR was developed for the rapid detection of genes encoding Shiga toxins 1 and 2 (stx1 and stx2), intimin (eaeA), and enterohemolysin A (hlyA) in 444 fecal samples derived from healthy and clinically affected cattle, sheep, pigs, and goats. The method involved non-solvent-based extraction of nucleic acid from an aliquot of an overnight culture of feces in EC (modified) broth. The detection limit of the assay for both fecal samples and pure cultures was between 18 and 37 genome equivalents. stx1 and hlyA were the most commonly encountered virulence factors.

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Year:  1999        PMID： 9925634      PMCID： PMC91113     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  28 in total

1.  Isolation of Escherichia coli serotype O157:H7 and other Shiga-like-toxin-producing E. coli from dairy cattle.

Authors:  J G Wells; L D Shipman; K D Greene; E G Sowers; J H Green; D N Cameron; F P Downes; M L Martin; P M Griffin; S M Ostroff
Journal:  J Clin Microbiol       Date:  1991-05       Impact factor: 5.948

Review 2.  Shiga and Shiga-like toxins.

Authors:  A D O'Brien; R K Holmes
Journal:  Microbiol Rev       Date:  1987-06

3.  Detection of Escherichia coli O157:H7 by multiplex PCR.

Authors:  P M Fratamico; S K Sackitey; M Wiedmann; M Y Deng
Journal:  J Clin Microbiol       Date:  1995-08       Impact factor: 5.948

4.  Frequent loss of Shiga-like toxin genes in clinical isolates of Escherichia coli upon subcultivation.

Authors:  H Karch; T Meyer; H Rüssmann; J Heesemann
Journal:  Infect Immun       Date:  1992-08       Impact factor: 3.441

5.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

6.  Polymerase chain reaction for diagnosis of enterohemorrhagic Escherichia coli infection and hemolytic-uremic syndrome.

Authors:  M J Brian; M Frosolono; B E Murray; A Miranda; E L Lopez; H F Gomez; T G Cleary
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

7.  A virulence plasmid in Escherichia coli enterotoxigenic for humans: intergenetic transfer and expression.

Authors:  T Yamamoto; T Honda; T Miwatani; T Yokota
Journal:  J Infect Dis       Date:  1984-11       Impact factor: 5.226

8.  Rapid and sensitive method for detection of Shiga-like toxin-producing Escherichia coli in ground beef using the polymerase chain reaction.

Authors:  V P Gannon; R K King; J Y Kim; E J Thomas
Journal:  Appl Environ Microbiol       Date:  1992-12       Impact factor: 4.792

9.  Virulence markers of Shiga-like toxin-producing Escherichia coli strains originating from healthy domestic animals of different species.

Authors:  L Beutin; D Geier; S Zimmermann; H Karch
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948

10.  Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933.

Authors:  H Schmidt; L Beutin; H Karch
Journal:  Infect Immun       Date:  1995-03       Impact factor: 3.441
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  58 in total

1.  Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes.

Authors:  T Bellin; M Pulz; A Matussek; H G Hempen; F Gunzer
Journal:  J Clin Microbiol       Date:  2001-01       Impact factor: 5.948

2.  Rapid detection of Shiga toxin-producing bacteria in feces by multiplex PCR with molecular beacons on the smart cycler.

Authors:  Simon D Bélanger; Maurice Boissinot; Christian Ménard; François J Picard; Michel G Bergeron
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

3.  Virulence properties and serotypes of Shiga toxin-producing Escherichia coli from healthy Australian cattle.

Authors:  Michael A Hornitzky; Barbara A Vanselow; Keith Walker; Karl A Bettelheim; Bruce Corney; Paul Gill; Graham Bailey; Steven P Djordjevic
Journal:  Appl Environ Microbiol       Date:  2002-12       Impact factor: 4.792

4.  Multiplex PCR assay for the detection of five putative virulence genes encoded in verotoxigenic Escherichia coli plasmids.

Authors:  A V Bustamante; A M Sanso; P M A Lucchesi; A E Parma
Journal:  Curr Microbiol       Date:  2011-01-29       Impact factor: 2.188

5.  Prevalence of Escherichia coli O157 in Saskatchewan cattle: characterization of isolates by using random amplified polymorphic DNA PCR, antibiotic resistance profiles, and pathogenicity determinants.

Authors:  Sinisa Vidovic; Darren R Korber
Journal:  Appl Environ Microbiol       Date:  2006-06       Impact factor: 4.792

6.  Virulence properties and serotypes of Shiga toxin-producing Escherichia coli from healthy Australian slaughter-age sheep.

Authors:  S P Djordjevic; M A Hornitzky; G Bailey; P Gill; B Vanselow; K Walker; K A Bettelheim
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

7.  Association of Escherichia coli O157:H7 with houseflies on a cattle farm.

Authors:  Muhammad J Alam; Ludek Zurek
Journal:  Appl Environ Microbiol       Date:  2004-12       Impact factor: 4.792

8.  Extended-spectrum beta-lactamase-producing Shiga toxin gene (Stx1)-positive Escherichia coli O26:H11: a new concern.

Authors:  Yoshikazu Ishii; Soichiro Kimura; Jimena Alba; Katsuaki Shiroto; Masanobu Otsuka; Naotaka Hashizume; Kazumichi Tamura; Keizo Yamaguchi
Journal:  J Clin Microbiol       Date:  2005-03       Impact factor: 5.948

9.  Differences in levels of secreted locus of enterocyte effacement proteins between human disease-associated and bovine Escherichia coli O157.

Authors:  A McNally; A J Roe; S Simpson; F M Thomson-Carter; D E Hoey; C Currie; T Chakraborty; D G Smith; D L Gally
Journal:  Infect Immun       Date:  2001-08       Impact factor: 3.441

10.  Detection and characterization of verocytotoxin-producing Escherichia coli by automated 5' nuclease PCR assay.

Authors:  Eva Møller Nielsen; Marianne Thorup Andersen
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948
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