Literature DB >> 11136804

Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes.

T Bellin1, M Pulz, A Matussek, H G Hempen, F Gunzer.   

Abstract

In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes about 45 min.

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Year:  2001        PMID： 11136804      PMCID： PMC87735          DOI： 10.1128/JCM.39.1.370-374.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  The LightCycler: a microvolume multisample fluorimeter with rapid temperature control.

Authors:  C T Wittwer; K M Ririe; R V Andrew; D A David; R A Gundry; U J Balis
Journal:  Biotechniques       Date:  1997-01       Impact factor: 1.993

2.  Nucleotide sequence analysis of the structural genes for Shiga-like toxin I encoded by bacteriophage 933J from Escherichia coli.

Authors:  M P Jackson; J W Newland; R K Holmes; A D O'Brien
Journal:  Microb Pathog       Date:  1987-02       Impact factor: 3.738

3.  Molecular microbiological investigation of an outbreak of hemolytic-uremic syndrome caused by dry fermented sausage contaminated with Shiga-like toxin-producing Escherichia coli.

Authors:  A W Paton; R M Ratcliff; R M Doyle; J Seymour-Murray; D Davos; J A Lanser; J C Paton
Journal:  J Clin Microbiol       Date:  1996-07       Impact factor: 5.948

Review 4.  Illnesses associated with Escherichia coli O157:H7 infections. A broad clinical spectrum.

Authors:  P M Griffin; S M Ostroff; R V Tauxe; K D Greene; J G Wells; J H Lewis; P A Blake
Journal:  Ann Intern Med       Date:  1988-11-01       Impact factor: 25.391

5.  Escherichia coli O157 infection disaster in Japan, 1996.

Authors:  H Yukioka; S Kurita
Journal:  Eur J Emerg Med       Date:  1997-09       Impact factor: 2.799

Review 6.  Escherichia coli O157:H7.

Authors:  P S Mead; P M Griffin
Journal:  Lancet       Date:  1998-10-10       Impact factor: 79.321

Review 7.  Escherichia coli O157:H7 and its significance in foods.

Authors:  M P Doyle
Journal:  Int J Food Microbiol       Date:  1991-04       Impact factor: 5.277

8.  Hemorrhagic colitis associated with a rare Escherichia coli serotype.

Authors:  L W Riley; R S Remis; S D Helgerson; H B McGee; J G Wells; B R Davis; R J Hebert; E S Olcott; L M Johnson; N T Hargrett; P A Blake; M L Cohen
Journal:  N Engl J Med       Date:  1983-03-24       Impact factor: 91.245

9.  Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction.

Authors:  H Karch; T Meyer
Journal:  J Clin Microbiol       Date:  1989-12       Impact factor: 5.948

Review 10.  Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections.

Authors:  J C Paton; A W Paton
Journal:  Clin Microbiol Rev       Date:  1998-07       Impact factor: 26.132
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  26 in total

1.  Quantitative real-time polymerase chain reaction for enteropathogenic Escherichia coli: a tool for investigation of asymptomatic versus symptomatic infections.

Authors:  Francesca Barletta; Theresa J Ochoa; Erik Mercado; Joaquim Ruiz; Lucie Ecker; Giovanni Lopez; Monica Mispireta; Ana I Gil; Claudio F Lanata; Thomas G Cleary
Journal:  Clin Infect Dis       Date:  2011-10-25       Impact factor: 9.079

2.  Multiplex real-time PCR for detection of Campylobacter, Salmonella, and Shigella.

Authors:  F Barletta; E H Mercado; A Lluque; J Ruiz; T G Cleary; T J Ochoa
Journal:  J Clin Microbiol       Date:  2013-06-12       Impact factor: 5.948

3.  A sensitive multiplex, real-time PCR assay for prospective detection of Shiga toxin-producing Escherichia coli from stool samples reveals similar incidences but variable severities of non-O157 and O157 infections in northern California.

Authors:  Martina I Lefterova; Kathleen A Slater; Indre Budvytiene; Patricia A Dadone; Niaz Banaei
Journal:  J Clin Microbiol       Date:  2013-07-10       Impact factor: 5.948

4.  Rapid and specific detection of Mycobacterium tuberculosis from acid-fast bacillus smear-positive respiratory specimens and BacT/ALERT MP culture bottles by using fluorogenic probes and real-time PCR.

Authors:  Nancimae Miller; Tim Cleary; Günter Kraus; Andrea K Young; Gina Spruill; H James Hnatyszyn
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

5.  New immuno-PCR assay for detection of low concentrations of shiga toxin 2 and its variants.

Authors:  Wenlan Zhang; Martina Bielaszewska; Matthias Pulz; Karsten Becker; Alexander W Friedrich; Helge Karch; Thorsten Kuczius
Journal:  J Clin Microbiol       Date:  2008-02-13       Impact factor: 5.948

6.  Rapid detection of Klebsiella pneumoniae from blood culture bottles by real-time PCR.

Authors:  Prathiba Kurupati; Carol Chow; Gamini Kumarasinghe; Chit Laa Poh
Journal:  J Clin Microbiol       Date:  2004-03       Impact factor: 5.948

7.  Multiplex fluorogenic real-time PCR for detection and quantification of Escherichia coli O157:H7 in dairy wastewater wetlands.

Authors:  A Mark Ibekwe; Pamela M Watt; Catherine M Grieve; Vijay K Sharma; Steven R Lyons
Journal:  Appl Environ Microbiol       Date:  2002-10       Impact factor: 4.792

8.  Comparison between O serotyping method and multiplex real-time PCR to identify diarrheagenic Escherichia coli in Taiwan.

Authors:  Ji-Rong Yang; Fang-Tzy Wu; Jin-Lai Tsai; Jung-Jung Mu; Ling-Fen Lin; Kuang-Lo Chen; Steve Hsu-Sung Kuo; Chuen-Sheue Chiang; Ho-Sheng Wu
Journal:  J Clin Microbiol       Date:  2007-08-29       Impact factor: 5.948

9.  Duplex real-time SYBR green PCR assays for detection of 17 species of food- or waterborne pathogens in stools.

Authors:  Hiroshi Fukushima; Yoshie Tsunomori; Ryotaro Seki
Journal:  J Clin Microbiol       Date:  2003-11       Impact factor: 5.948

10.  Real-time fluorescence PCR assays for detection and characterization of Shiga toxin, intimin, and enterohemolysin genes from Shiga toxin-producing Escherichia coli.

Authors:  Udo Reischl; Mohammad T Youssef; Jochen Kilwinski; Norbert Lehn; Wen Lan Zhang; Helge Karch; Nancy A Strockbine
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948
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