Literature DB >> 9925626

Evaluation of immunomagnetic separation for recovery of infectious Cryptosporidium parvum oocysts from environmental samples.

P A Rochelle1, R De Leon, A Johnson, M H Stewart, R L Wolfe.   

Abstract

Two commercial immunomagnetic separation (IMS) kits for Cryptosporidium were compared for recovery of oocysts from environmental samples. Oocyst recovery efficiencies with the Dynal and Crypto-Scan kits ranged from 62 to 100% and 34 to 74%, respectively, for seeded environmental water concentrates (turbidity of 210 to 11,480 nephelometric turbidity units). Recovery efficiencies were dependent on the mechanism of agitation during the magnetic capture procedure. An assay combining in vitro cell culture and reverse transcriptase PCR demonstrated that oocysts recovered by IMS retained their infectivity.

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Year:  1999        PMID： 9925626      PMCID： PMC91105     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  15 in total

1.  Comparison of three stool-processing methods for detection of Salmonella serogroups B, C2, and D by PCR.

Authors:  U Kongmuang; J M Luk; A A Lindberg
Journal:  J Clin Microbiol       Date:  1994-12       Impact factor: 5.948

2.  Detection of hepatitis A virus in environmental samples by antigen-capture PCR.

Authors:  M Y Deng; S P Day; D O Cliver
Journal:  Appl Environ Microbiol       Date:  1994-06       Impact factor: 4.792

3.  Comparison of two methods for detection of Giardia cysts and Cryptosporidium oocysts in water.

Authors:  E C Nieminski; F W Schaefer; J E Ongerth
Journal:  Appl Environ Microbiol       Date:  1995-05       Impact factor: 4.792

4.  Antibody-magnetite method for selective concentration of Giardia lamblia cysts from water samples.

Authors:  J M Bifulco; F W Schaefer
Journal:  Appl Environ Microbiol       Date:  1993-03       Impact factor: 4.792

5.  Anti-Cryptosporidium parvum antibodies inhibit infectivity in vitro and in vivo.

Authors:  P S Doyle; J Crabb; C Petersen
Journal:  Infect Immun       Date:  1993-10       Impact factor: 3.441

6.  Kinetics of Cryptosporidium parvum sporozoite neutralization by monoclonal antibodies, immune bovine serum, and immune bovine colostrum.

Authors:  L E Perryman; M W Riggs; P H Mason; R Fayer
Journal:  Infect Immun       Date:  1990-01       Impact factor: 3.441

7.  Immunomagnetic separation and PCR for detection of Helicobacter pylori in water and stool specimens.

Authors:  H Enroth; L Engstrand
Journal:  J Clin Microbiol       Date:  1995-08       Impact factor: 5.948

8.  Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples.

Authors:  D W Johnson; N J Pieniazek; D W Griffin; L Misener; J B Rose
Journal:  Appl Environ Microbiol       Date:  1995-11       Impact factor: 4.792

9.  Evaluation of the immunofluorescence procedure for detection of Giardia cysts and Cryptosporidium oocysts in water.

Authors:  M W LeChevallier; W D Norton; J E Siegel; M Abbaszadegan
Journal:  Appl Environ Microbiol       Date:  1995-02       Impact factor: 4.792

10.  Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes.

Authors:  A T Campbell; L J Robertson; H V Smith
Journal:  Appl Environ Microbiol       Date:  1992-11       Impact factor: 4.792
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  16 in total

1.  Comparison of sensitivity of immunofluorescent microscopy to that of a combination of immunofluorescent microscopy and immunomagnetic separation for detection of Cryptosporidium parvum oocysts in adult bovine feces.

Authors:  M D Pereira; E R Atwill; T Jones
Journal:  Appl Environ Microbiol       Date:  1999-07       Impact factor: 4.792

2.  Effects of pH and magnetic material on immunomagnetic separation of Cryptosporidium oocysts from concentrated water samples.

Authors:  Ryan C Kuhn; Channah M Rock; Kevin H Oshima
Journal:  Appl Environ Microbiol       Date:  2002-04       Impact factor: 4.792

3.  Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors.

Authors:  Jianlin Jiang; Kerri A Alderisio; Ajaib Singh; Lihua Xiao
Journal:  Appl Environ Microbiol       Date:  2005-03       Impact factor: 4.792

4.  Performance and specificity of the covalently linked immunomagnetic separation-ATP method for rapid detection and enumeration of enterococci in coastal environments.

Authors:  Amity G Zimmer-Faust; Vanessa Thulsiraj; Donna Ferguson; Jennifer A Jay
Journal:  Appl Environ Microbiol       Date:  2014-02-21       Impact factor: 4.792

5.  Effect of particles on the recovery of cryptosporidium oocysts from source water samples of various turbidities.

Authors:  Yao Yu Feng; Say Leong Ong; Jiang Yong Hu; Lian Fa Song; Xiao Lan Tan; Wun Jern Ng
Journal:  Appl Environ Microbiol       Date:  2003-04       Impact factor: 4.792

6.  Quantitative-PCR assessment of Cryptosporidium parvum cell culture infection.

Authors:  George D Di Giovanni; Mark W LeChevallier
Journal:  Appl Environ Microbiol       Date:  2005-03       Impact factor: 4.792

7.  Optimization of a reusable hollow-fiber ultrafilter for simultaneous concentration of enteric bacteria, protozoa, and viruses from water.

Authors:  Hugo A Morales-Morales; Guadalupe Vidal; John Olszewski; Channah M Rock; Debanjana Dasgupta; Kevin H Oshima; Geoffrey B Smith
Journal:  Appl Environ Microbiol       Date:  2003-07       Impact factor: 4.792

8.  Recovery and enumeration of Cryptosporidium parvum from animal fecal matrices.

Authors:  Cheryl M Davies; Christine Kaucner; Daniel Deere; Nicholas J Ashbolt
Journal:  Appl Environ Microbiol       Date:  2003-05       Impact factor: 4.792

9.  Detection and genotyping of oocysts of Cryptosporidium parvum by real-time PCR and melting curve analysis.

Authors:  Sultan Tanriverdi; Atila Tanyeli; Fikri Başlamişli; Fatih Köksal; Yurdanur Kilinç; Xiaochuan Feng; Glenda Batzer; Saul Tzipori; Giovanni Widmer
Journal:  J Clin Microbiol       Date:  2002-09       Impact factor: 5.948

10.  Improved risk analysis by dual direct detection of total and infectious Cryptosporidium oocysts on cell culture in combination with immunofluorescence assay.

Authors:  Cindy Lalancette; George D Di Giovanni; Michèle Prévost
Journal:  Appl Environ Microbiol       Date:  2009-11-20       Impact factor: 4.792
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