OBJECTIVE: The study of the antioxidant status in female adolescents (N = 82) with anorexia nervosa, by the measurement of erythrocyte tocopherol concentration, and the determination of activities of the main antioxidant enzymes: superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase. METHOD: Tocopherol was measured by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection, and antioxidant enzyme activities by spectrometric methods using a Cobas Fara automated centrifugal analyzer. RESULTS: Tocopherol was significantly decreased in the anorexic patients compared to reference values (p < .02). In 21% of patients, tocopherol levels were below the reference interval. Superoxide dismutase activity was significantly decreased (p < .0001), while catalase activity was increased (p < .0001). The activity of the glutathione system enzymes did not show significant differences between patients and controls. DISCUSSION: The deficient concentration of erythrocyte tocopherol together with the altered antioxidant enzyme activities suggest a certain degree of oxidative damage in anorexia nervosa owing to both factors deficient micronutrient intake and oxidative stress.
OBJECTIVE: The study of the antioxidant status in female adolescents (N = 82) with anorexia nervosa, by the measurement of erythrocyte tocopherol concentration, and the determination of activities of the main antioxidant enzymes: superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase. METHOD:Tocopherol was measured by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection, and antioxidant enzyme activities by spectrometric methods using a Cobas Fara automated centrifugal analyzer. RESULTS:Tocopherol was significantly decreased in the anorexic patients compared to reference values (p < .02). In 21% of patients, tocopherol levels were below the reference interval. Superoxide dismutase activity was significantly decreased (p < .0001), while catalase activity was increased (p < .0001). The activity of the glutathione system enzymes did not show significant differences between patients and controls. DISCUSSION: The deficient concentration of erythrocyte tocopherol together with the altered antioxidant enzyme activities suggest a certain degree of oxidative damage in anorexia nervosa owing to both factors deficient micronutrient intake and oxidative stress.
Authors: Arianna Vignini; Paola Canibus; Laura Nanetti; Giorgio Montecchiani; Emanuela Faloia; Anna Maria Cester; Marco Boscaro; Laura Mazzanti Journal: Neuromolecular Med Date: 2007-09-15 Impact factor: 3.843
Authors: E Agnello; G Malfi; A M Costantino; P Massarenti; M Pugliese; N Fortunati; M G Catalano; A Palmo Journal: Eat Weight Disord Date: 2012-09 Impact factor: 4.652
Authors: Victor M Victor; Susana Rovira-Llopis; Vanessa Saiz-Alarcon; Maria C Sangüesa; Luis Rojo-Bofill; Celia Bañuls; Rosa Falcón; Raquel Castelló; Luis Rojo; Milagros Rocha; Antonio Hernández-Mijares Journal: PLoS One Date: 2014-09-25 Impact factor: 3.240