Literature DB >> 9920791

Transfer of sulfur to biotin from biotin synthase (BioB protein).

K J Gibson1, D A Pelletier, I M Turner.   

Abstract

In biotin synthase reactions carried out in vitro, we observed efficient transfer of 35S to biotin from partially purified E. coli biotin synthase (product of the bioB gene) labelled by biosynthetic incorporation of [35S]-cysteine. Mass spectrometry was consistent with covalent alteration of the protein in the assay. These results suggest that BioB protein is a reagent, not a catalyst. Copyright 1999 Academic Press.

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Year:  1999        PMID： 9920791     DOI： 10.1006/bbrc.1998.9991

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  16 in total

1.  Evidence from Mössbauer spectroscopy for distinct [2Fe-2S](2+) and [4Fe-4S](2+) cluster binding sites in biotin synthase from Escherichia coli.

Authors:  Natalia B Ugulava; Kristene K Surerus; Joseph T Jarrett
Journal:  J Am Chem Soc       Date:  2002-08-07       Impact factor: 15.419

2.  Spectroscopic changes during a single turnover of biotin synthase: destruction of a [2Fe-2S] cluster accompanies sulfur insertion.

Authors:  N B Ugulava; C J Sacanell; J T Jarrett
Journal:  Biochemistry       Date:  2001-07-27       Impact factor: 3.162

3.  Biotin synthase contains two distinct iron-sulfur cluster binding sites: chemical and spectroelectrochemical analysis of iron-sulfur cluster interconversions.

Authors:  N B Ugulava; B R Gibney; J T Jarrett
Journal:  Biochemistry       Date:  2001-07-27       Impact factor: 3.162

4.  Role of the [2Fe-2S]2+ cluster in biotin synthase: mutagenesis of the atypical metal ligand arginine 260.

Authors:  Robyn B Broach; Joseph T Jarrett
Journal:  Biochemistry       Date:  2006-11-28       Impact factor: 3.162

5.  9-Mercaptodethiobiotin is formed as a competent catalytic intermediate by Escherichia coli biotin synthase.

Authors:  Andrew M Taylor; Christine E Farrar; Joseph T Jarrett
Journal:  Biochemistry       Date:  2008-08-09       Impact factor: 3.162

6.  The Arabidopsis Bio2 protein requires mitochondrial targeting for activity.

Authors:  Nadège Arnal; Claude Alban; Martine Quadrado; Olivier Grandjean; Hakim Mireau
Journal:  Plant Mol Biol       Date:  2006-08-01       Impact factor: 4.076

7.  Reduction of the [2Fe-2S] cluster accompanies formation of the intermediate 9-mercaptodethiobiotin in Escherichia coli biotin synthase.

Authors:  Andrew M Taylor; Stefan Stoll; R David Britt; Joseph T Jarrett
Journal:  Biochemistry       Date:  2011-08-25       Impact factor: 3.162

Review 8.  Anaerobic functionalization of unactivated C-H bonds.

Authors:  Squire J Booker
Journal:  Curr Opin Chem Biol       Date:  2009-03-16       Impact factor: 8.822

9.  Loss of iron-sulfur clusters from biotin synthase as a result of catalysis promotes unfolding and degradation.

Authors:  Michael R Reyda; Rachael Dippold; Michael E Dotson; Joseph T Jarrett
Journal:  Arch Biochem Biophys       Date:  2007-12-10       Impact factor: 4.013

10.  A combined computational and experimental investigation of the [2Fe-2S] cluster in biotin synthase.

Authors:  Michael G G Fuchs; Franc Meyer; Ulf Ryde
Journal:  J Biol Inorg Chem       Date:  2009-09-19       Impact factor: 3.358
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