Literature DB >> 9915494

Expression profiling using cDNA microarrays.

D J Duggan1, M Bittner, Y Chen, P Meltzer, J M Trent.   

Abstract

cDNA microarrays are capable of profiling gene expression patterns of tens of thousands of genes in a single experiment. DNA targets, in the form of 3' expressed sequence tags (ESTs), are arrayed onto glass slides (or membranes) and probed with fluorescent- or radioactively-labelled cDNAs. Here, we review technical aspects of cDNA microarrays, including the general principles, fabrication of the arrays, target labelling, image analysis and data extraction, management and mining.

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Year:  1999        PMID： 9915494     DOI： 10.1038/4434

Source DB:  PubMed          Journal:  Nat Genet        ISSN： 1061-4036            Impact factor:   38.330


  231 in total

Review 1.  The complexity of radiation stress responses: analysis by informatics and functional genomics approaches.

Authors:  A J Fornace; S A Amundson; M Bittner; T G Myers; P Meltzer; J N Weinsten; J Trent
Journal:  Gene Expr       Date:  1999

Review 2.  Mutational analysis using oligonucleotide microarrays.

Authors:  J G Hacia; F S Collins
Journal:  J Med Genet       Date:  1999-10       Impact factor: 6.318

3.  Profiling and verification of gene expression patterns in normal and malignant human prostate tissues by cDNA microarray analysis.

Authors:  H Chaib; E K Cockrell; M A Rubin; J A Macoska
Journal:  Neoplasia       Date:  2001 Jan-Feb       Impact factor: 5.715

4.  Informatic selection of a neural crest-melanocyte cDNA set for microarray analysis.

Authors:  S K Loftus; Y Chen; G Gooden; J F Ryan; G Birznieks; M Hilliard; A D Baxevanis; M Bittner; P Meltzer; J Trent; W Pavan
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-03       Impact factor: 11.205

Review 5.  Microbial relatives of the seed storage proteins of higher plants: conservation of structure and diversification of function during evolution of the cupin superfamily.

Authors:  J M Dunwell; S Khuri; P J Gane
Journal:  Microbiol Mol Biol Rev       Date:  2000-03       Impact factor: 11.056

6.  Normalization strategies for cDNA microarrays.

Authors:  J Schuchhardt; D Beule; A Malik; E Wolski; H Eickhoff; H Lehrach; H Herzel
Journal:  Nucleic Acids Res       Date:  2000-05-15       Impact factor: 16.971

7.  Anopheles gambiae pilot gene discovery project: identification of mosquito innate immunity genes from expressed sequence tags generated from immune-competent cell lines.

Authors:  G Dimopoulos; T L Casavant; S Chang; T Scheetz; C Roberts; M Donohue; J Schultz; V Benes; P Bork; W Ansorge; M B Soares; F C Kafatos
Journal:  Proc Natl Acad Sci U S A       Date:  2000-06-06       Impact factor: 11.205

8.  Expression profiling by iAFLP: A PCR-based method for genome-wide gene expression profiling.

Authors:  S Kawamoto; T Ohnishi; H Kita; O Chisaka; K Okubo
Journal:  Genome Res       Date:  1999-12       Impact factor: 9.043

9.  cDNA array analysis of altered gene expression in human endothelial cells in response to Chlamydia pneumoniae infection.

Authors:  B K Coombes; J B Mahony
Journal:  Infect Immun       Date:  2001-03       Impact factor: 3.441

10.  Apoptosis signals in atopy and asthma measured with cDNA arrays.

Authors:  M H Brutsche; I C Brutsche; P Wood; A Brass; N Morrison; M Rattay; N Mogulkoc; N Simler; M Craven; A Custovic; J J Egan; A Woodcock
Journal:  Clin Exp Immunol       Date:  2001-02       Impact factor: 4.330
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