Literature DB >> 10773095

Normalization strategies for cDNA microarrays.

J Schuchhardt1, D Beule, A Malik, E Wolski, H Eickhoff, H Lehrach, H Herzel.   

Abstract

Multiple Arabidopsis thaliana clones from an experimental series of cDNA microarrays are evaluated in order to identify essential sources of noise in the spotting and hybridization process. Theoretical and experimental strategies for an improved quantitative evaluation of cDNA microarrays are proposed and tested on a series of differently diluted control clones. Several sources of noise are identified from the data. Systematic and stochastic fluctuations in the spotting process are reduced by control spots and statistical techniques. The reliability of slide to slide comparison is critically assessed within the statistical framework of pattern matching and classification.

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Year:  2000        PMID： 10773095      PMCID： PMC105386          DOI： 10.1093/nar/28.10.e47

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

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Review 5.  Hybridization analyses of arrayed cDNA libraries.

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  60 in total

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Journal:  Nucleic Acids Res       Date:  2001-12-01       Impact factor: 16.971

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5.  Tissue gene expression analysis using arrayed normalized cDNA libraries.

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9.  Microarray optimizations: increasing spot accuracy and automated identification of true microarray signals.

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10.  Analysis of repeatability in spotted cDNA microarrays.

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