Literature DB >> 9889304

Surface charge response of the phosphatidylcholine head group in bilayered micelles from phosphorus and deuterium nuclear magnetic resonance.

K J Crowell1, P M Macdonald.   

Abstract

Solid-state phosphorus (31P) and deuterium (2H) nuclear magnetic resonance (NMR) spectroscopy over the temperature range of 25-50 degreesC were used to investigate bilayered micelles (bicelles) composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1, 2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) in the presence of either the anionic lipid 1,2-dimyristoyl-sn-3-phosphoglycerol (DMPG) or the cationic lipid 1,2-dimyristoyl-3-trimethylammonium-propane (DMTAP). The 31P-NMR spectra demonstrate that bicellar structures form with DMPG/DMPC ratios ranging from 0 to 50/50 and with DMTAP/DMPC ratios from 0 to 40/60, while the overall concentration of DHPC remains constant. The formation of bicelles containing charged amphiphiles is contingent upon the presence of NaCl, with 50 mM NaCl being sufficient for bicelle formation at all concentrations of charged amphiphile investigated, while 150 mM NaCl affords better resolution of the various 31P-NMR resonance signals. The 2H-NMR spectra demonstrate that the quadrupolar splittings (Deltanu) of head group-deuterated DMPC change inversely as a function of the amount of negative versus positive charge present, and that the changes for deuterons on the alpha-carbon are opposite in sense to those for deuterons on the beta-carbon. This indicates that head group-deuterated phosphatidylcholine functions as a molecular voltmeter in bicelles in much the same fashion as it does in spherical vesicles.

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Year:  1999        PMID: 9889304     DOI: 10.1016/s0005-2736(98)00206-5

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN: 0006-3002


  14 in total

1.  An efficient (1)H/(31)P double-resonance solid-state NMR probe that utilizes a scroll coil.

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Journal:  J Magn Reson       Date:  2007-08-06       Impact factor: 2.229

2.  Amphipathic antimicrobial piscidin in magnetically aligned lipid bilayers.

Authors:  Anna A De Angelis; Christopher V Grant; Matthew K Baxter; Jason A McGavin; Stanley J Opella; Myriam L Cotten
Journal:  Biophys J       Date:  2011-09-07       Impact factor: 4.033

3.  Influence of the long-chain/short-chain amphiphile ratio on lateral diffusion of PEG-lipid in magnetically aligned lipid bilayers as measured via pulsed-field-gradient NMR.

Authors:  Ronald Soong; Peter M Macdonald
Journal:  Biophys J       Date:  2005-07-01       Impact factor: 4.033

4.  Ionizable Nitroxides for Studying Local Electrostatic Properties of Lipid Bilayers and Protein Systems by EPR.

Authors:  Maxim A Voinov; Alex I Smirnov
Journal:  Methods Enzymol       Date:  2015-09-09       Impact factor: 1.600

5.  Europium III binding and the reorientation of magnetically aligned bicelles: insights from deuterium NMR spectroscopy.

Authors:  K J Crowell; P M Macdonald
Journal:  Biophys J       Date:  2001-07       Impact factor: 4.033

6.  Spin-labeled pH-sensitive phospholipids for interfacial pKa determination: synthesis and characterization in aqueous and micellar solutions.

Authors:  Maxim A Voinov; Igor A Kirilyuk; Alex I Smirnov
Journal:  J Phys Chem B       Date:  2009-03-19       Impact factor: 2.991

Review 7.  Spatial reorientation experiments for NMR of solids and partially oriented liquids.

Authors:  Rachel W Martin; John E Kelly; Kelsey A Collier
Journal:  Prog Nucl Magn Reson Spectrosc       Date:  2015-10-23       Impact factor: 9.795

8.  Surface electrostatics of lipid bilayers by EPR of a pH-sensitive spin-labeled lipid.

Authors:  Maxim A Voinov; Izarys Rivera-Rivera; Alex I Smirnov
Journal:  Biophys J       Date:  2013-01-08       Impact factor: 4.033

9.  Lateral diffusion of PEG-Lipid in magnetically aligned bicelles measured using stimulated echo pulsed field gradient 1H NMR.

Authors:  Ronald Soong; Peter M Macdonald
Journal:  Biophys J       Date:  2004-10-08       Impact factor: 4.033

10.  Interaction of the neuropeptide met-enkephalin with zwitterionic and negatively charged bicelles as viewed by 31P and 2H solid-state NMR.

Authors:  Isabelle Marcotte; Erick J Dufourc; Marise Ouellet; Michèle Auger
Journal:  Biophys J       Date:  2003-07       Impact factor: 4.033

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