Literature DB >> 988782

Radioimmunoassay of iodinated tobramycin.

A Broughton, J E Strong, L K Pickering, G P Bodney.   

Abstract

A radioimmunoassay for tobramycin was developed using antisera from rabbits injected with a tobramycin-bovine serum albumin conjugate. The antibody had a relatively high affinity for (125)I-labeled tobramycin (1.6 x 10(9) liters/mol), thus indicating little interference in immunoreactivity by iodination. The radioimmunoassay was highly specific, and only minor cross-reaction occurred with other aminoglycoside antibiotics. The radioimmunoassay was also more sensitive than previously published tobramycin assays (minimum sensitivity, 280 pg), and there was a significant correlation (P < 0.05) with both the radioenzymatic and microbiological assays.

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Year:  1976        PMID： 988782      PMCID： PMC429809          DOI： 10.1128/AAC.10.4.652

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  15 in total

1.  Improved acetylating radioenzymatic assay of amikacin, tobramycin, and sisomicin in serum.

Authors:  P Stevens; L S Young; W L Hewitt
Journal:  Antimicrob Agents Chemother       Date:  1975-03       Impact factor: 5.191

2.  Gentamicin blood levels: a guide to nephrotoxicity.

Authors:  J G Dahlgren; E T Anderson; W L Hewitt
Journal:  Antimicrob Agents Chemother       Date:  1975-07       Impact factor: 5.191

3.  A rapid chemical assay for gentamicin.

Authors:  D H Smith; B Van Otto; A L Smith
Journal:  N Engl J Med       Date:  1972-03-16       Impact factor: 91.245

4.  Clinical and laboratory evidence for inactivation of gentamicin by carbenicillin.

Authors:  J E McLaughlin; D S Reeves
Journal:  Lancet       Date:  1971-02-06       Impact factor: 79.321

5.  Radioimmunoassay, acetylating radio-enzymatic assay, and microbioassay of gentamicin: a comparative study.

Authors:  P Stevens; L S Young; W L Hewitt
Journal:  J Lab Clin Med       Date:  1975-08

6.  Biological aspects of the interaction between gentamicin and carbenicillin.

Authors:  J A Waitz; C G Drube; E L Moss; E M Oden; J V Bailey
Journal:  J Antibiot (Tokyo)       Date:  1972-04       Impact factor: 2.649

7.  ANTIBODIES TO BRADYKININ AND ANGIOTENSIN: A USE OF CARBODIIMIDES IN IMMUNOLOGY.

Authors:  T L GOODFRIEND; L LEVINE; G D FASMAN
Journal:  Science       Date:  1964-06-12       Impact factor: 47.728

8.  The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.

Authors:  A E Bolton; W M Hunter
Journal:  Biochem J       Date:  1973-07       Impact factor: 3.857

9.  Experience in monitoring gentamicin therapy during treatment of serious gram-negative sepsis.

Authors:  P Noone; T M Parsons; J R Pattison; R C Slack; D Garfield-Davies; K Hughes
Journal:  Br Med J       Date:  1974-03-16

10.  Gentamicin:adenine mononucleotide transferase: partial purification, characterization, and use in the clinical quantitation of gentamicin.

Authors:  A L Smith; D H Smith
Journal:  J Infect Dis       Date:  1974-04       Impact factor: 5.226
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  11 in total

1.  Comparison of the latex agglutination inhibition assay for tobramycin with radioimmunoassay.

Authors:  S D Sears; H C Standiford; D Bernstein; H C Nipper; B A Tatem; U Smalls; E Caplan
Journal:  Eur J Clin Microbiol       Date:  1986-06       Impact factor: 3.267

2.  Assay of serum tobramycin levels with the Bactec 460.

Authors:  L E Phillips; R R Martin; L O Gentry; T E Rogers
Journal:  Antimicrob Agents Chemother       Date:  1979-10       Impact factor: 5.191

3.  Comparative efficacies of piperacillin, azlocillin, ticarcillin, aztreonam, and tobramycin against experimental Pseudomonas aeruginosa pneumonia.

Authors:  J B Schiff; J E Pennington
Journal:  Antimicrob Agents Chemother       Date:  1984-01       Impact factor: 5.191

4.  Comparison of radioimmunoassay with a new immunofluorescent method (FIAX) for measuring tobramycin in serum.

Authors:  D A Bruckner; J A Hindler; W J Martin; R Palmer
Journal:  Antimicrob Agents Chemother       Date:  1982-01       Impact factor: 5.191

5.  Rapid, reproducible enzyme immunoassay for tobramycin.

Authors:  E L Francke; S Srinivasan; P Labthavikul; H C Neu
Journal:  J Clin Microbiol       Date:  1981-01       Impact factor: 5.948

6.  Comparison of a radioimmunoassay and a microbiological assay for measurement of serum vancomycin concentrations.

Authors:  K B Crossley; J C Rotschafer; M M Chern; K E Mead; D E Zaske
Journal:  Antimicrob Agents Chemother       Date:  1980-04       Impact factor: 5.191

7.  New schedule for tobramycin administration.

Authors:  R D Lawson; G P Bodey; T Pan; T L Smith
Journal:  Antimicrob Agents Chemother       Date:  1980-05       Impact factor: 5.191

Review 8.  Why monitor serum levels of gentamicin?

Authors:  M Barza; M Lauermann
Journal:  Clin Pharmacokinet       Date:  1978 May-Jun       Impact factor: 6.447

9.  Comparative activities of N-formimidoyl thienamycin, ticarcillin, and tobramycin against experimental Pseudomonas aeruginosa pneumonia.

Authors:  J E Pennington; C E Johnson
Journal:  Antimicrob Agents Chemother       Date:  1982-09       Impact factor: 5.191

10.  Effect of time and concentration upon interaction between gentamicin, tobramycin, Netilmicin, or amikacin and carbenicillin or ticarcillin.

Authors:  L K Pickering; P Gearhart
Journal:  Antimicrob Agents Chemother       Date:  1979-04       Impact factor: 5.191
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