| Literature DB >> 9885563 |
H Ge1, Y Si, A P Wolffe.
Abstract
Increasing evidence suggests that pre-mRNA splicing can take place cotranscriptionally in vivo. However, insight into how these two processes are linked has been lacking. Here, we describe that a novel transcriptional coactivator, p52, interacts not only with transcriptional activators and general transcription factors to enhance activated transcription but also with the essential splicing factor ASF/SF2 both in vitro and in vivo to modulate ASF/SF2-mediated pre-mRNA splicing. Furthermore, immunofluorescence studies indicate that the majority of endogenous p52 is colocalized with ASF/SF2 in the nucleoplasm of HeLa cells. Together, these observations suggest that, in addition to functioning as a transcriptional coactivator, p52 may also act as an adaptor to coordinate pre-mRNA splicing and transcriptional activation of class II genes.Entities:
Mesh：
Substances：
Year: 1998 PMID： 9885563 DOI： 10.1016/s1097-2765(00)80290-7
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970